利用低温放热法分析板栗枝条的抗寒性

doi:10.7668/hbnxb.2017.S1.035

摘要/Abstract

摘要： 为验证低温放热（LTE）法鉴定板栗枝条抗寒性的应用效果，以16个板栗品种（系）休眠期枝条为材料，采用LTE法分析各品种放热特征和LTE相关指标，三点法建立各品种枝条木质部和韧皮部的温度-伤害度回归方程（LT-I），利用LTE相关指标（木质部和韧皮部LT-I斜率、木质部和韧皮部的开始结冰和终止结冰点温度）结合隶属函数法分析了16个品种的抗寒性，并用传统组织褐变法和电导法对LTE分析法效果进行验证。结果表明：16个板栗品种（系）枝条木质部和韧皮部的LT-I斜率在品种间和品种内均不同，并且各品种LTE相关指标均达到显著差异水平。板栗枝条LTE各指标隶属度均值与木质部和韧皮部的开始结冰和终止结冰点温度呈极显著负相关。LTE分析法测定木质部伤害度和组织褐变法测定木质部褐变率呈显著正相关，相关系数为0.988，LTE分析法测定枝条伤害度和电导法测定枝条伤害度呈极显著正相关，相关系数为0.998。因此，LTE分析法是一种简单准确实用的鉴定板栗枝条抗寒性的方法。板栗枝条木质部和韧皮部开始结冰和终止结冰点温度越低，抗寒性隶属度均值越大，其抗寒性越强。

关键词: 板栗, 枝条, 抗寒性, 低温放热分析法

Abstract: The experiment was to verify the effect of low temperature exotherms analysis on identifying the cold resistance of chestnut.The test materials were annual dormant branches of 16 chestnut varieties(strain) from 8 provinces,the exothermic characteristics of each varieties and the correlation index of LTE were analyzed by LTE analysis method.Low temperature exotherms(LTE) of 16 chestnut varieties(strain) were recorded by differential thermal analysis(DTA),the regression line of injury-temperature(LT-I) of xylem and phloem were established by three-point method,the LTE correlation index(LT-I slope of xylem and phloem,the temperature of freeze beginning and freeze ending point of xylem and phloem) and the membership function method were applied to compare cold hardiness of 16 chestnut varieties(strain).Tissue browning and conductivity method were used to validated the correctness of low temperature exotherms analysis method.The LT-I slope of phloem was different from xylem,the LT-I slope of xylem and phloem were different among 16 chestnut varieties,and the LTE correlation indexes had significant differences.There was highly significant negative correlation between the average membership of LTE index and the temperature of freeze beginning and freeze ending point of xylem and phloem.There was significant positive correlation between xylem injury degree determined by LTE analysis method and xylem browning rate determined by tissue browning,and the correlation coefficient was 0.988.There was highly significant positive correlation between branch injury degree determined by LTE analysis method and branch injury degree determined by conductometry,and the correlation coefficient was 0.998.The results show that LTE analysis is a simple,accurate and practical method for identifying the cold resistance of Chinese chestnut.The lower temperature of freeze beginning and freeze ending point of xylem and phloem,and the bigger average subordination value of LTE indexes in the chestnut branches is,the more cold resistance capacity the chestnut variety has.

Key words: Chinese chestnut, Branch, Cold resistance, Low temperature exotherms analysis

中图分类号:

S664.2

郭燕, 李颖, 张树航, 张馨方, 王广鹏. 利用低温放热法分析板栗枝条的抗寒性[J]. 华北农学报, 2017, 32(S1): 201-209. doi: 10.7668/hbnxb.2017.S1.035.

GUO Yan, LI Ying, ZHANG Shuhang, ZHANG Xinfang, WANG Guangpeng. Using Low Temperature Exotherms Method to Analyze Branch Cold Resistance of Chinese Chestnut[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(S1): 201-209. doi: 10.7668/hbnxb.2017.S1.035.

http://www.hbnxb.net/CN/Y2017/V32/IS1/201

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