四种树木端粒酶活性与树龄及叶位的相关性

doi:10.7668/hbnxb.2014.S1.038

华北农学报 ›› 2014, Vol. 29 ›› Issue (S1): 197-201. doi: 10.7668/hbnxb.2014.S1.038

四种树木端粒酶活性与树龄及叶位的相关性

张徐俞¹, 王瑾瑜², 郑广顺³, 张俊琦¹, 卢存福¹

1. 北京林业大学生物学院, 分析测试中心, 林木育种国家工程实验室, 北京 100083;
2. 清华大学分析测试中心, 北京 100084;
3. 中国科学院植物研究所, 北京 100093

收稿日期:2014-10-17 出版日期:2014-12-28
通讯作者: 卢存福(1966-),男,北京海淀人,教授,博士,主要从事植物分子细胞生物学研究。
作者简介:张徐俞(1990-),女,浙江衢州人,在读硕士,主要从事植物端粒、端粒酶的研究。
基金资助:
高等学校学科创新引智计划资助项目(B13007);长江学者和创新团队发展计划资助项目(IRT13047);北京市教委共建项目(No.101009);北京市公园管理中心课题项目(No.0710016);北京市自然科学基金项目(No.6112016)

Analysis of Age- and Leaf Position-associated Telomerase Activity in Four Tree Species

ZHANG Xu-yu¹, WANG Jin-yu², ZHENG Guang-shun³, ZHANG Jun-qi¹, LU Cun-fu¹

1. College of Biological Sciences and Biotechnology, Analysis and Testing Center, National Engineering Laboratory for Tree Breeding, Beijing Forestry University, Beijing 100083, China;
2. Analysis and Testing Center, Tsinghua University, Beijing 100083, China;
3. Institute of Botany, Chinese Academy of Sciences, Beijing 100093, China

Received:2014-10-17 Published:2014-12-28

摘要/Abstract

摘要： 为探究端粒酶活性与树龄以及叶位是否存在一定的关系,应用TRAP法对不同年龄的油松、银杏、楸树、国槐叶片及油松和银杏同一棵树不同叶位的叶片进行端粒酶活性的测定。结果表明,油松端粒酶活性同树龄无明确的相关性,而楸树、国槐的端粒酶活性随着树龄增加而表现为增加的趋势。端粒酶活性与叶位相关,越靠近顶端的叶片端粒酶活性较高,而靠近下端的叶片端粒酶活性较低。

关键词: 树龄, 端粒, 端粒酶, 叶位

Abstract: The aim of this study was to determine the variation of telomerase during the life-span and with sampling position of leaves in different tree species.Therefore,TRAP assay was used to determine telomerase activity in Pinus tabulaeformis, Ginkgo biloba, Catalpa bungei C.A.Mey,and Sophora japonica Linn.The results showed that no significant difference of telomerase activity was found among different ages of trees.But the higher telomerase activitives were found in the top position leaves of Pinus tabulaeformis and Ginkgo biloba.The effect of leaf position on the telomerase activity in Pinus tabulaeformis and Ginkgo biloba was strong and significant.This is the first observation on such a position-related variation in telomerase activity.

Key words: Tree age, Telomere, Telomerase, Leaf position

中图分类号:

张徐俞, 王瑾瑜, 郑广顺, 张俊琦, 卢存福. 四种树木端粒酶活性与树龄及叶位的相关性[J]. 华北农学报, 2014, 29(S1): 197-201. doi: 10.7668/hbnxb.2014.S1.038.

ZHANG Xu-yu, WANG Jin-yu, ZHENG Guang-shun, ZHANG Jun-qi, LU Cun-fu. Analysis of Age- and Leaf Position-associated Telomerase Activity in Four Tree Species[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(S1): 197-201. doi: 10.7668/hbnxb.2014.S1.038.

http://www.hbnxb.net/CN/Y2014/V29/IS1/197

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