摘要： 克隆了与草莓成熟有关的乙烯受体Ers1基因片段,为进一步研究Ers1基因功能并通过基因技术改善草莓贮运性能奠定基础。以全明星草莓成熟果实中分离得到基因组DNA为模板,经PCR扩增到一条约700 bp的特异片段,将该片段克隆到PEGM-T easy vector上经测序分析,基因全长667 bp,编号222个氨基酸。序列分析结果表明,该序列与Chandler-Ers1的cDNA序列同源性99%,氨基酸序列同源性98%。

关键词: 草莓, 乙烯受体Ers1, 基因克隆, 序列分析

Abstract: In this study a ripening related ethvlene recepor Ers1 gene in strawberry fruits was cloned for the following-up reseach on gene and improving varieties of strawberry through transgenic technology.A pair of primers were designed and synthesized based on the sequence reported of Chandler-Ers1.Using genome DNA extracted from All-star strawberry mature fruits as template the specific PCR product of Ers1 was obtained,then it was ligated to pEGM-T easy vector and sequenced.The sequencing data showed that the PCR product was 667 bp encoding 222 peedicted amino acid residues.Comparison with the cDNA sequence from Chandler-Ers1 indicated the homlogy was 98%,and amino acid identitise were 97%.

Key words: Strawberry, Ethylene receptor Ers1, Gene clone, Sequence analysis

中图分类号: 

• S668.4