黄瓜Cs-ppc基因的组织表达分析及光强对其在叶片中转录水平的影响

doi:10.7668/hbnxb.2011.05.005

华北农学报 ›› 2011, Vol. 26 ›› Issue (5): 21-24. doi: 10.7668/hbnxb.2011.05.005

所属专题： 黄瓜；生物技术；

黄瓜Cs-ppc基因的组织表达分析及光强对其在叶片中转录水平的影响

郭芳, 谭峥, 刘兴旺, 刘丽英, 任华中

中国农业大学农学与生物技术学院蔬菜系, 北京 100193

收稿日期:2011-08-11 出版日期:2011-10-28
通讯作者: 任华中( 1963-) ，男，山东人，教授，主要从事黄瓜遗传与栽培生理研究。
作者简介:郭芳( 1985-) ，女，河北人，硕士研究生，主要从事黄瓜生理与分子生物学研究。
基金资助:
“973”项目(2009CB11900);科技支撑项目(2009BADB8B02;2011BAD12B03);中央高校基本科研业务费专项资金项目(15050702);果类蔬菜产业技术体系北京市创新团队项目

Transcriptional Analyzing of Cs-ppcGenes and Their Response to Light Intensity in Cucumber Leaves

GUO Fang, TAN Zheng, LIU Xing-wang, LIU Li-ying, REN Hua-zhong

College of Agronomy & Biotechnology, China Agricultural University, Beijing 100193, China

Received:2011-08-11 Published:2011-10-28

摘要/Abstract

摘要： 为了解黄瓜中ppc基因在各组织的转录水平及不同光强对叶片中该家族基因转录水平的影响,本研究对已克隆得到的Cs-ppc1、Cs-ppc2和Cs-ppc3基因的转录水平进行荧光定量PCR分析。Cs-ppc1和Cs-ppc3在多个组织中的表达量均较高,Cs-ppc2基因的组织表达差异性较大,花中的表达量高而在其他组织中的表达量均很低。对光强的响应中,Cs-ppc1的表达量及生物周期受光强的影响比其他两个Cs-ppc基因明显。Cs-ppc2基因的组织表达差异性较大,可能在花发育过程中发挥重要作用,而推测Cs-ppc1在适应不同光强调节的过程中发挥着重要作用。

关键词: 黄瓜, PEPC, Cs-ppc基因, 光强, 表达分析

Abstract: To study the transcription level of ppc genes in organizations of cucumber,and influences of light intensity on them in leaves,we used fluorescence quantitative PCR to detect the transcription level three cucumber ppc genes.It showed that the transcription level of Cs-ppc1 and Cs-ppc3 in many tissues were all high,while its level of Cs-ppc2 was very high in flowers and low in other tissues.By contrast,the effect of light intensity on Cs-ppc1 transcription was more obvious than the other ppc genes.Cs-ppc2′s high transcription level in flowers suggest its function in floral development,while Cs-ppc1 may play an important role in the adaption to different light intensity.

Key words: Cucumber, Phosphoenolpyruvate carboxylase, Gs-ppc genes, Light intensity, Transcription analysis

中图分类号:

郭芳, 谭峥, 刘兴旺, 刘丽英, 任华中. 黄瓜Cs-ppc基因的组织表达分析及光强对其在叶片中转录水平的影响[J]. 华北农学报, 2011, 26(5): 21-24. doi: 10.7668/hbnxb.2011.05.005.

GUO Fang, TAN Zheng, LIU Xing-wang, LIU Li-ying, REN Hua-zhong. Transcriptional Analyzing of Cs-ppcGenes and Their Response to Light Intensity in Cucumber Leaves[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2011, 26(5): 21-24. doi: 10.7668/hbnxb.2011.05.005.

http://www.hbnxb.net/CN/Y2011/V26/I5/21

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黄瓜Cs-ppc基因的组织表达分析及光强对其在叶片中转录水平的影响

Transcriptional Analyzing of Cs-ppcGenes and Their Response to Light Intensity in Cucumber Leaves

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