摘要： 白藜芦醇是植物遭受胁迫时自身分泌的一种可抵御病菌感染的抗菌素,对真菌具有一定的抑制作用.而白藜芦醇合成酶在白藜芦醇合成途径中具有关键性的作用.水稻稻瘟病作为一种由真菌引起的严重病害,具有高发性、毁灭性的特点,对水稻品质及生产危害极大.本研究从花生中分离到白藜芦醇合酶基因PNRSC,通过基因工程的方法构建入经改造的以玉米泛素Ubi为启动子、以生物安全性的bar基因为选择标记的植物双元表达载体pCAMBIA1300中,经农杆菌介导对黄淮稻区主栽品种圣稻13进行遗传转化,经Basta抗性鉴定及分子筛选,证明白藜芦醇合成酶基因已整合入水稻基因组,并获得转基因株系.目前转基因植株已移栽至大田,为进一步进行基因功能鉴定及田间抗病水稻新品种培育提供了候选材料.

关键词: 白藜芦醇, 白藜芦醇合成酶基因PNRSC, 水稻稻瘟病, Ubi启动子, bar基因, Basta, 抗病水稻新品种培育

Abstract: Resveratrol is a kind of phytoalexins which carl defence pathogen infection when suffered stresses.And resveratrol synthase,a kind of rate-limiting enzymes in Res synthesis pathway,plays key role during Res synthesis procedure.Rice blast,which caused by Magnaporthe grisea,is an extremely hazardous and widely distributed fun-gus disease.In this study,eDNA of resveratrol synthase gene was isolated using peanut total RNA as template by RTPCR amplification.The plant expression binary vector of RS was then constructed using modified pCAMBIAl300 as framework plasmid which using maize ubiquitin promoter and biosecurity screening marker bar instead of 35S promoter and Hygromycin by genetic engineering.The mature embyro of rice Shengdao 13 were transformed by the recombi· nated vector through agrobacterium-mediated transformation,and the positive transformants were further screened by Basta and PCR analysis.The work above will provided new materials for RS gene function identification,and shed some light for new rice germplasm innovation of disease resistence in the future.

Key words: Resveratrol(Res), Resveratrol synthase gene PNRSC, Rice blast, Ubiquitin promoter(Ubi), bar gene, Basta, Cultivation of new germplasm of rice disease resistance

中图分类号: 

• Q78