摘要： 根据已知的NBS-LRR类抗病基因结构中氨基酸的保守区域，设计简并引物，通过RT-PCR扩增及克隆，从东乡野生稻(DongxiangOryzarufipogonGriff.)中共获得7个新的NBS-LRR类抗病基因同源片段。所有7个抗病基因同源序列均含有NBS-LRR类抗病基因的保守序列，如P-loop、Kinase2、Kinase3a以及跨膜区域等。通过氨基酸同源性比较和分析，发现克隆到的抗病基因同源序列与已克隆的水稻白叶枯抗病基因Xa1的相应氨基酸序列存在约40%的同源性，其中的3个NBS抗病基因同源序列还与小麦抗叶锈病基因Lr1存在40%的同源性。

关键词: 东乡野生稻, 抗病基因, RT-PCR, NBS-LRR同源序列

Abstract: Degenerate oligonucleotide primers were designed on the basis of nucleotide-binding site(NBS) motifsconserved among the reported disease resistance genes. By reverse transcription polymerise chain reaction(RT-PCR)，seven new NBS,RR disease resistance gene analogues hive been obtained and cloned from Dongxiing Oryarufgon Griff. Sequencing of the DNA fragments indicated that ill of NBS resistance gene analogues contained theconserved motifs of NBS,RR type resistance genes such is Poop ( Kinise 1 a)，Kinise 2，Kinise 3i and trinsmem-brince domain.

Key words: Donxian Oryza rujoon Griff, Diseaseesistance gene, Reverse transcription polymerise chain re-action(RTCR), Nucleotide-binding site (NBS) eucineich repeit (LRR)

中图分类号: 

• S511.9