乳蛋白糖基化修饰研究进展

doi:10.7668/hbnxb.2010.S2.012

摘要/Abstract

摘要： 蛋白质糖基化是一种重要的翻译后修饰。蛋白质糖基化研究主要集中于检测糖基化位点,以及连接在该位点的聚糖结构。笔者综述了蛋白质糖基化的特性,富集和相关检测技术,及运用糖基化分析手段研究乳蛋白糖基化位点和位点的不均一性,以进一步阐述翻译后修饰造成乳蛋白的多态性。

关键词: 乳蛋白, 糖基化, 质谱

Abstract: "Glycosylation is one of the most common post-translational modifications of proteins.Glycoprotein analysis requires determining both the sites of glycosylation as well as the glycan structures associated with each site.In this context,advance in the detection technologies of glycoprotein and the development of protein glycosylation modification,proteomics with mass spectrometry were extensively to obtain the glycosylation site and the site microheterogeneity of milk protein,and further for exploring polymorphism of milk protein. "

Key words: M ilk prote in, Glycosy lation, Mass spectrometry

中图分类号:

S852.23

李珊珊, 王加启, 杨永新, 魏宏阳, 卜登攀, 周凌云. 乳蛋白糖基化修饰研究进展[J]. 华北农学报, 2010, 25(S2): 49-52. doi: 10.7668/hbnxb.2010.S2.012.

LI Shan-shan, WANG Jia-qi, YANG Yong-xin, WEI Hong-yang, BU Deng-pan, ZHOU Ling-yun. Advances in the Glycosylation of Milk Protein[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(S2): 49-52. doi: 10.7668/hbnxb.2010.S2.012.

http://www.hbnxb.net/CN/Y2010/V25/IS2/49

参考文献

[1] Apweiler R,Hermjakob H,Sharon N.On the frequency of protein glycosylation,as deduced from analysis of the SWISS-PROT database[J].Biochim Biophys Acta,1999,1473(1):4-8.
[2] Schweppe R E,Haydon C E,Lewis T S,et al.The characterization of protein post-translational modifications by mass spectrometry[J].Acc Chem Res,2003,36:453-461.
[3] Wormald M R,Sharon N.Carbohydrates and glycoconjugates hot roles for glycosylation:signal transduetion,control of cell development and differentiation,and innate immunity[J].Current Opinion in Structural Biololgy,2001,12:567-568.
[4] Helenius A,Aebi M.Intracellular functions of N-linked glycans[J].Science,2001,291:2364-2369.
[5] 代景泉,蔡耘,钱小红.蛋白质糖基化分析方法及其在蛋白质组学中的应用[J].生物技术通讯,2005,16(3):287-292.
[6] 朱立平.蛋白质糖基化与B细胞免疫[J].上海免疫学杂志,2001,21(4):193-194.
[7] Wei X,Li L.Comparative glycoproteomics:approaches and applications[J].Brief Funct Genomic Roteomic,2008,8(2):104-113.
[8] spiro R G.Protein glycosylation:nature,distribution,enzymatic formation,and disease implications of glyeopeptide bonds[J].Glycobiology,2002,12(4):43R-56R.
[9] An H J,Froehlich J W,Lebrilla C B.Determination of glyeosylation sites and site-specific heterogeneity in glycoproteins[J].Current Opinion in Chemical Biology,2009,13:421-426.
[10] Zhang H,Aebersold R.Isolation of glycoproteins and identification of their N-linkod glycosylation sites[J].Methods Mol Biol,2006,328:177-185.
[11] Sparbier K,Koch S,Kessler I,et al.Selective isolation of glycoproteins and glycopeptides for MALDI-TOF MS detection supported by magnetic particles[J].J Biomol Tech,2005,16:407-413.
[12] Alvarez-Manilla G,Atwood J,Cuo Y,et al.Tools for glycoproteomic analysis:size exclusion chromatography facilitates identification of tryptic glycopeptides with Nlinked glyeosylation sites[J].J Proteome Bes,2006,5:701-708.
[13] Takegawa Y,Ito H,Keira T,et al.Profiling of N-and Oglycopeptides of erythropoietin by capillary zwitterionic type of hydrophilic interaction chromatography/electrospray ionization mass spectrometry[J].J Sep Sci,2008,31:1585-1593.
[14] Kaji H,Saito H,Yamauehi Y,et al.Lectin affinity capture,isotope-coded tagging and mass spectrometry to identify N-linked glycoproteins[J].Nat Biotech,2003,21:667-672.
[15] Davies M J,Hounsell E F.HPLC and HPAEC of oligosaceharides and glycopeptides[J].Methods Mol Biol 1998,76:79-100.
[16] Harvey D J.Proteomic analysis of glyeosylation structural determination of N-and O-linked glycans by mass spectrometry[J].Expert Bey Protcomics,2005,2(1):87-101.
[17] Mirgorodskaya E,Hassan H,Wandall H H,et al.Partial vaporphase hydrolysis of peptide bonds:a method for mass spectrometric determination of O-glycosylated sites in glyeopeptides[J].Anal.Biochem,1999,269:54-65.
[18] Gerken T A,Owens C L,Pasumarthy M.Determination of the site-specific O-glyeosylation pattern of the porcine submaxillary muein tandem repeat glycopeptide.Model proposed for the polypeptide:GalNAe transferase peptide binding site[J].J Biol Chem,1997,272:9709-9719.
[19] Holland J W,Deeth H C,Alewood P F.Proteomic analysis of K-casein miero-heterogeneity[J].Proteomics,2004,4:743-752.
[20] Charlwood J,Skehel J M,Camilleri P.Analysis of Nlinked oligosaeeharides released from glyeoproteins separated by two-dcmensional gel electrophoresis[J].Anal Biochem,2000,284(1):49-59.
[21] Charlwood J,Hanrahan S,Tyldesley R,et al.Use of proteomic methodology for the characterization of human milk fat globular membrane proteins[J].Analytical Biochemistry,2002,301(2):314-324.
[22] Chen W,Lee P J,Stapels M,et al.The use of mass spectrometry to determine location and extent of N-glycosylation on folate binding protein from bovine milk[J].Rapid Commun Mass Speetrom,2006,20(2):313-316.
[23] Hagglund P,Bunkenborg J,Elortza F,et al.A new strategy for identification of N-glyeosylated proteins and unambiguoas assignment of their glyeosylation sites using HILIC enrichment and partial deglycosylation[J].J Proteome Res,2004,3(3):556-566.
[24] Viseux N,Hironowski X,Delaney J,et al.Qualitative and quantitative analysis of the glycosylation pattern of recombinant proteins[J].Anal Chem,2001,73:4755-4762.
[25] Mamone G,Caira S,Carro G,et al.Casein phosphoproteome:Identification of phosphoproteins by combined mass spectrometry and two-dimensional gel electropboresis[J].Electrophoresis,2003,24:2824-2837.
[26] Inagaki M,Nakaya S,Nohara D,et al.The muhiplicity of N-glycan structures of bovine milk 18 kda lactophorin(milk GlyCAM-1)[J].Biosci Bioteehnol Biochem,2010,74(2):447-450.
[27] Pisano A,Packer N H,Redmond J W,et al.Characterization of O-linked glycosylation motifs in the glycopeptide domain of bovine kappa-casein[J].Glycobiology,1994,4(6):837-844.
[28] Moreno F J,Recio I,Olano A,et al.Chromatographic characterization of ovine kappa-casein maeropeptide[J].J Dairy Res,2000,67(3):349-359.
[29] Moreno F J,Recio l,Olano A,et al.Heterogeneity of caprine kappa-casein maeropeptide[J].J Dairy Res,2001,68(2):197-208.
[30] Cebo C,Caillat H,Bouvier F.Major proteins of the goat milk fat globule membrane[J].J Dairy Sci,2010,93(3):868-876.
[31] Holland J W,Deeth H C,Alewood P F.Analysis of Oglycosylation site occupancy in bovine k-casein glycoforms separated by two-dimensional gel electrophoresis[J].Proteomics,2005,5:990-1002.
[32] Froehlich J W,Dodds E D,Barboza M.Glycoprotein Expression in Human Milk during Lactation[J].J Agric Food Chem,2010,58:6440-6448.

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