大白菜遗传图谱的构建及与染色体关联分析

doi:10.7668/hbnxb.2010.03.019

华北农学报 ›› 2010, Vol. 25 ›› Issue (3): 80-86. doi: 10.7668/hbnxb.2010.03.019

所属专题： 白菜；

大白菜遗传图谱的构建及与染色体关联分析

原玉香¹, 张晓伟¹, 孙日飞², 王晓武², 张慧², 蒋武生¹, 姚秋菊¹, 耿建峰¹

1. 河南省农业科学院, 园艺研究所, 河南郑州 450002;
2. 中国农业科学院, 蔬菜花卉研究所, 北京 100081

收稿日期:2010-02-13 出版日期:2010-06-28
通讯作者: 张晓伟(1965－)，男，河南平玉人，研究员，主要从事蔬菜遗传育种研究。
作者简介:原玉香(1970-),女,河南武陟人,副研究员,博士,主要从事大白菜生物技术遗传育种研究.
基金资助:
国家“863”计划项目(2008AA10Z148);“十一五”国家科技支撑计划子课题(2006BAD01A7-1-06)

Construction of a Genetic Linkage Map Anchoring to Chromosomes of Chinese Cabbage

YUAN Yu-xiang¹, ZHANG Xiao-wei¹, SUN Ri-fei², WANG Xiao-wu², ZHANG Hui², JIANG Wu-sheng¹, YAO Qiu-ju¹, GENG Jian-feng¹

1. Institute of Horticulture, Henan Academy of Agricultural Sciences, Zhengzhou 450002, China;
2. Institute of Vegetables and Flowers, Chinese Academy of Agriculture Sciences, Beijing 100081, China

Received:2010-02-13 Published:2010-06-28

摘要/Abstract

摘要： 为构建大白菜遗传图谱,并与国际上公认的白菜参照图谱相关联。以大白菜晚抽薹DH系Y177-12和早抽薹DH系Y195-93为亲本建立的183个DH系为作图群体,利用SRAP、SSR、AFLP、STS、ESTP和CAPS等多种分子标记和形态标记构建大白菜遗传图谱,通过参照图上的锚定标记确定连锁群,并用最新的连锁群命名法进行命名。构建了包含10个连锁群、233个标记位点的分子遗传图谱,其中包括145个SRAP标记、62个SSR标记、12个AFLP标记、6个STS标记、4个ESTP标记、3个形态标记和1个CAPS标记。图谱总长度1 036 cM,标记间的平均图距为4.4cM。通过其中的38个SSR,4个ESTP和2个STS标记将各个连锁群与国际上认可A1-A10的参照连锁群相关联。该图谱的构建将为不同图谱的比较与整合及重要农艺性状的基因定位奠定基础。

关键词: 大白菜, 分子标记, 遗传图谱, 染色体关联

Abstract: The objective of the present study is to construct a genetic linkage map associated with internationally agreed reference linkage map in Chinese cabbage ( Brassica rapa L.ssp.pekinensis).183 DH lines derived from a cross of two DH lines,Y177-12 and Y195-93,were used for map construction.Six kinds of marker systems including SRAP, SSR,AFLP, STS,ESTP and CAPS loci were used for polymorphic marker screening.The assignment of linkage groups to their chromosomes was determined by using markers which have been mapped in the reference map.A genetic linkage map was constructed for Chinese cabbage, covering 1 036 cM,with an average mapping interval of 4.4 cM.233 loci including 145 SRAP, 62 SSR, 12 AFLP,6 STS,4 ESTP,1 CAPS and 3 morphological markers were integrated into 10 linkage groups using JoinMap 3.0.38 SSRs,4 ESTPs and 2 STSs provided anchors to previously published linkage map for B.rapa and B.napus and the consensus nomenclature of A1-A10 was adopted in this study.A genetic linkage map of Chinese cabbage associated with chromosomes was constructed,which enabled the alignments and integration between sets of maps and populations and would be useful in gene localization, comparative genomes research and QTL mapping of important agronomic traits for Chinese cabbage．

Key words: Chinese cabbage, Molecular marker, Genetic linkage map, Chromosome assignment

中图分类号:

S634.03

原玉香, 张晓伟, 孙日飞, 王晓武, 张慧, 蒋武生, 姚秋菊, 耿建峰. 大白菜遗传图谱的构建及与染色体关联分析[J]. 华北农学报, 2010, 25(3): 80-86. doi: 10.7668/hbnxb.2010.03.019.

YUAN Yu-xiang, ZHANG Xiao-wei, SUN Ri-fei, WANG Xiao-wu, ZHANG Hui, JIANG Wu-sheng, YAO Qiu-ju, GENG Jian-feng. Construction of a Genetic Linkage Map Anchoring to Chromosomes of Chinese Cabbage[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 80-86. doi: 10.7668/hbnxb.2010.03.019.

http://www.hbnxb.net/CN/Y2010/V25/I3/80

参考文献

[1] Song K M,Suzuki J Y,Solcum M K,et al.A linkage map of Brassica rapa (syn.campestris) based on restriction fragment length polymorphism loci[J].Theor Appl Genet,1991,82:296-304.
[2] Chyi Y S,Hoenecke M E,Sernyk J L.A genetic map of restriction fragment length polymorphism loci for Brassica rapa (syn.campestris)[J].Genetics,1992,138:963-391.
[3] Teutonico R A,Osborn T C.Mapp ing of RFLP and qualitative trait loci in Brassica rapa and comparison to the linkage maps of B.napus,B.oleracea and Arabidopsis thaliana[J].Theor Appl Genet,1994,89:885-894.
[4] Ajisaka H,Kuginuki Y,Hida K,et al.A linkage map of DNA markers in Brassica campestris[J].Breed Sci,1995,45(Suppl.):195.
[5] Kole C,Kole P,Vogelzang R,et al.Genetic linkage map of a Brassica rapa recombinant inbred population[J].J Hered,1997,88:553-557.
[6] Matsumoto E,Yasui C,Ohi M,et al.Linkage analysis of RFLP markers for clubroot resistance and pigmentation in Chinese cabbage (Brassica rapa ssp.pekinensis)[J].Euphytica,1998,104(2):79-86.
[7] Moriguch K.A genetic map based on RAPD,RFLP,isozyme,morphological markers and QTL analysis for clubroot resistance in Brassica oleracea[J].Breeding Science,1999,49(4):257-265.
[8] Nozaki T,Kumazaki A,Koba T,et al.Linkage analysis among loci for RAPDs,isozymes and some agronomic traits in Brassica campestris L[J].Euphytica,1997,95:115-123.
[9] 张鲁刚,王鸣,陈杭,等.中国白菜RAPD分子连锁图谱的构建[J].植物学报,2000,42(5):485-489.
[10] 于拴仓,王永健,郑晓鹰.大白菜分子遗传图谱的构建与分析[J].中国农业科学,2003,36(2):190-195.
[11] 王美,张凤兰,孟祥栋,等.中国白菜AFLP分子遗传图谱的构建[J].华北农学报,2004,19(1):28-33.
[12] 张晓芬,王晓武,娄平,等.利用大白菜DH群体构建AFLP遗传连锁图谱[J].园艺学报,2005,32(3):443-448.
[13] Zhang X W,Wu J,Zhao J J,et al.Identification of QTLs related to bolting in Brassica rapa ssp.pekinensis (syn.Brassica campestris ssp.pekinensis)[J].Agr Sci China,2006,5:265-271.
[14] 王淼,王剑,李宏博,等.利用抗、染根肿病F2群体构建大白菜AFLP遗传连锁图谱[J].华北农学报,2009,24(2):64-70
[15] 原玉香,张晓伟,蒋武生,等.芸薹属蔬菜分子育种研究进展[J].河南农业科学,2009(1):155-160.
[16] Suwabe K,Iketani H,Nunome T,et al.Isolation and characterization of microsatellites in Brassica rapa L[J].Theor Appl Genet,2002,104:1092-1098.
[17] Suwabe K,Iketani H,Nunome T,et al.Characteristics of microsatellites in Brassica rapa genome and their potential utilization for comparative genomics in Cruciferae[J].Breed Sci,2004,54:85-90.
[18] Lowe A J,Moule C,Trick M,et al.Efficient large-scale development of microsatellites for marker and mapping applications in Brassica crop species[J].Theor Appl Genet,2004,108:1103-1112.
[19] Suwabe K,Tsukazaki H,Iketani H,et al.Simple sequence repeat-based comparative genomics between Brassica rapa and Arabidopsis thaliana:the genetic origin of clubroot resistance[J].Genetics,2006,173:309-319.
[20] Kim J S,Chung T Y,King G J,et al.A sequence-tagged linkage map of Brassica rapa[J].Genetics,2006,174:29-39.
[21] Choi S R,Teakle G R,Plaha P,et al.The reference genetic linkage map for the multinational Brassica rapa genome sequencing project[J].Theor Appl Genet,2007,115(6):777-792.
[22] Wang X W,Lou P,Bonnema G,et al.Linkage mapping of a dominant male sterility gene Ms-cd1 in Brasscia oleracea[J].Genome,2005,48:848-854.
[23] Li G,Quiros C F.Sequence-related amplified polymorphism (SRAP),a new marker system based on a simple PCR reaction:its application in mapping and gene tagging in Brasisca[J].Theor Appl Genet,2001,103:455-461.
[24] Riaz A,Li G,Quresh Z,et al.Genetic diversity of oilseed Brassica napus inbred lines based on sequence-related amplified polymorphism and its relation to hybrid performance[J].Plant Breeding,2001,120:411-415.
[25] Budak H,Shearman R C,Parmaksiz I,et al.Molecular characterization of Buffalograss germplasm using sequence-related amplified polymorphism markers[J].Theor Appl Genet,2004,108:328-334.
[26] Lin Z,Zhang X,Nie Y,et al.Construction of a genetic linkage map for cotton based on SRAP[J].Chinese Science Bulletin,2003,48:2063-2067.
[27] 葛佳,谢华,崔崇士,等.大白菜表达序列标签SSR标记分析[J].农业生物技术学报,2005,13(4):423-428.
[28] Vos P,Hogers R,Bleeker M,et al.AFLP:a new technique for DNA fingerprinting[J].Nucleic Acids Res,1995,23:4407-4414.
[29] 原玉香,孙日飞,张晓伟,等.芸薹种作物抽薹相关基因BrFLC1的CAPS标记[J].园艺学报,2008,35(11):1635-1640.
[30] Kosambi D D.The estimation of map distance from recombination values[J].Ann Eugen,1944,12:172-175.

选择文件类型/文献管理软件名称

选择包含的内容

大白菜遗传图谱的构建及与染色体关联分析

Construction of a Genetic Linkage Map Anchoring to Chromosomes of Chinese Cabbage

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价

[1]	王佳荣, 董瑞, 张梦宇, 高璞, 张培培, 李在峰, 刘大群. 40份CIMMYT小麦品系苗期及成株抗叶锈病基因鉴定[J]. 华北农学报, 2022, 37(2): 201-210.
[2]	张辉, 王良, 付增娟, 李晓东, 赵尚敏, 鄂圆圆, 郑文哲, 张自强, 张必周, 张惠忠. VNTR特异性引物在甜菜育性鉴定中的应用[J]. 华北农学报, 2022, 37(2): 35-41.
[3]	李曼, 史晓蕾, 邸锐, 刘志芳, 孟庆民, 付才, 杨春燕, 王冬梅, 张孟臣, 张洁, 闫龙. 大豆长片段插入/缺失标记的开发与应用[J]. 华北农学报, 2022, 37(1): 18-26.
[4]	武悦, 陈阳, 王星哲, 单飞彪, 张勇, 孙鸿举. 扁茎黄芪转录组测序及生物信息学分析[J]. 华北农学报, 2022, 37(1): 42-49.
[5]	张红, 徐莹莉, 王超楠, 黄志银, 范伟强, 李梅, 张斌. 大白菜抗干烧心病分子标记的开发与验证[J]. 华北农学报, 2021, 36(6): 195-203.
[6]	吴晓军, 李奉原, 申云霞, 陈向东, 胡喜贵, 姜豪, 张雪宁, 李小利, 胡铁柱, 茹振钢. 基于小麦外引种质资源的粒质量基因分子标记检测及组合分析[J]. 华北农学报, 2021, 36(5): 127-134.
[7]	吴婷婷, 陈海龙, 黄俊, 梁毅, 张婷, 李博文, 赖怡帆, 邹玉莹, 车凡昊, 吴俊, 刘金灵, 刘雄伦. Pi9基因分子标记辅助选择改良水稻[J]. 华北农学报, 2021, 36(5): 191-197.
[8]	高广亮, 张克山, 许国洋, 赵献芝, 陈主平, 周莉, 王启贵. GnIH基因多态性与鹅产蛋和体组成性状的关联分析[J]. 华北农学报, 2021, 36(5): 204-210.
[9]	钟婷婷, 郭诗芬, 卢文斌, 肖钢. 甘蓝型油菜抗根肿病KASP标记开发和利用[J]. 华北农学报, 2021, 36(4): 184-190.
[10]	吴晓军, 胡喜贵, 陈向东, 姜小苓, 张雪宁, 王玉泉, 董娜, 胡铁柱, 李笑慧, 茹振钢. 基于小麦外引种质资源的抗旱性分子标记检测及实用性评价[J]. 华北农学报, 2021, 36(3): 74-82.
[11]	冯俊彦, 康乐, 郎涛, 张聪, 李明, 赵珊, 蒲志刚. 基于SCoT分子标记的甘薯及其野生种遗传多样性分析[J]. 华北农学报, 2021, 36(1): 18-26.
[12]	钮笑晓, 李小波, 孙毅, 马海林, 张洁, 刘亚飞, 郝曜山, 崔贵梅. 玉米茎腐(青枯)病研究进展[J]. 华北农学报, 2020, 35(S1): 433-440.
[13]	张帅, 张希兰, 张娜, 赵明辉, 乔文臣, 孙丽静, 李辉, 傅晓艺, 何明琦, 纪军, 李俊明. 黄淮麦区北片小麦穗部相关性状的全基因组关联分析[J]. 华北农学报, 2020, 35(S1): 31-39.
[14]	张浩, 柳絮, 宣宁, 张华, 高瑞钰, 赵倩倩, 姚方印. 分子标记辅助选择培育低直链淀粉含量抗除草剂水稻[J]. 华北农学报, 2020, 35(S1): 78-84.
[15]	孟雅宁, 严立斌, 张红肖, 赵海涛, 范妍芹. 辣椒抗疫病基因分子标记的筛选及检测[J]. 华北农学报, 2020, 35(S1): 338-341.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

大白菜遗传图谱的构建及与染色体关联分析

Construction of a Genetic Linkage Map Anchoring to Chromosomes of Chinese Cabbage

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 15

编辑推荐

Metrics

本文评价