45S rDNA基因在新麦草染色体上的分布

doi:10.7668/hbnxb.2010.03.002

华北农学报 ›› 2010, Vol. 25 ›› Issue (3): 5-8. doi: 10.7668/hbnxb.2010.03.002

所属专题： 生物技术；

45S rDNA基因在新麦草染色体上的分布

云岚¹, 云锦凤¹, 王秀娥², 李海凤², 方宇辉²

1. 内蒙古农业大学, 生态环境学院, 内蒙古呼和浩特 010018;
2. 南京农业大学, 农业部细胞遗传重点开放实验室, 江苏南京 210095

收稿日期:2009-12-11 出版日期:2010-06-28
通讯作者: 云锦凤(1942－)，女，内蒙古土默特左旗人，教授，博士生导师，主要从事牧草种质资源与遗传育种研究。
作者简介:云岚(1970-),女,内蒙古呼和浩特人,副教授,博士,主要从事牧草种质资源与遗传育种研究.
基金资助:
国家科技支撑计划项目(2008BADB3B03)

The Distribution of 45S rDNA in Chromosome of Russian Wild Ryegrass

YUN Lan¹, YUN Jin-feng¹, WANG Xiu-e², LI Hai-feng², FANG Yu-hui²

1. College of Ecological and Environmental Science, Inner Mongolia Agricultural University, Huhhot 010018, China;
2. Cytogenetic Lab of Nanjing Agricultural University, Nanjing 210095, China

Received:2009-12-11 Published:2010-06-28

摘要/Abstract

摘要： 分析rDNA基因位点在染色体上的分布可以对新麦草染色体进行识别和分析其基因组特征。利用FISH和顺序C-分带-FISH技术将45S rDNA定位于新麦草细胞分裂中期染色体上,结果表明,45S rDNA在二倍体新麦草染色体上有6个主要分布位点,另外几条染色体在两臂中部或长臂末端还显示出较弱的杂交信号,信号强度显示蒙农4号新麦草基因组具有一定杂合性。分析确定新麦草的45S rDNA基因主位点分别位于N1染色体短臂末端、N3染色体短臂末端以及N5染色体短臂末端,推测这3对染色体是NOR染色体。

关键词: 新麦草, 45S rDNA, C-分带, 荧光原位杂交

Abstract: Chromosome and genome of Russian wild rye could be analyzed by the rDNA gene location.Chromosome was analyzed by sequential C-banding and 45S rDNA FISH.There were 6 main 45S rDNA sites located in the end of short arms of 3 pairs of diploid chromosome.They were chromosome N1,N3 and N5,which can be speculated as NOR chromosome.There were also weak hybridization signal on some long arm of chromosome.Heterozygosity could be identified in Genome of variety Mengnong No.4 by hybridization signal strength．

Key words: Russian wild rye, 45S rDNA, C-banding, Fluorescence in situ hybridization

中图分类号:

S813.9

云岚, 云锦凤, 王秀娥, 李海凤, 方宇辉. 45S rDNA基因在新麦草染色体上的分布[J]. 华北农学报, 2010, 25(3): 5-8. doi: 10.7668/hbnxb.2010.03.002.

YUN Lan, YUN Jin-feng, WANG Xiu-e, LI Hai-feng, FANG Yu-hui. The Distribution of 45S rDNA in Chromosome of Russian Wild Ryegrass[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(3): 5-8. doi: 10.7668/hbnxb.2010.03.002.

http://www.hbnxb.net/CN/Y2010/V25/I3/5

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