摘要： 黄瓜枯萎病是造成黄瓜减产的主要病害之一,研究黄瓜枯萎病抗性分子机理对黄瓜抗病育种有重要意义.以黄瓜抗枯萎病材料Cu1为试材,采用基因差异显示技术(DDRT-PCR)研究黄瓜接种枯萎病生理小种后基因的表达情况,分离出1个接种后在根茎部特异表达的差异cDNA片断,命名为A178-2.A178-2基因片段的328个核苷酸中有15个核苷酸与拟南芥泛素蛋白同源,氨基酸序列同源性达到85%.

关键词: 黄瓜, 枯萎病生理小种, 抗病相关基因cDNA, 分离, 鉴定

Abstract: Fusarium wilt(Fusarium oxysporum f.sp.cucumerinum Owen.) is one of major diseases that cause significant disease losses in cucumber.It is significant to explore the disease resistance molecular mechanism against Fusarium wilt race 4 in cucumber breeding.The cucumber resistant line Cu14 were employed for studying on resistant genes expressing against Fusarium wilt by differential display reverse transcriptase PCR(DDRT-PCR) method.One fragment of cDNA expressed specially was got from the root-stem inoculated in cucumber,and was named as A178-2 with the size of 328 bp.Blast analysis showed that 145 bp nucleotide sequences of A178-2 was homologous with the generic grain protein in Arabidopsis and reached 85%.Further studying on expression of the gene will be done.

Key words: Cucumber, Fusarium wilt race 4, Disease resistance-related genes, Isolation, Identification

中图分类号: 

• Q74

• S642.2

• S436.421