类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响

doi:10.7668/hbnxb.2009.S1.001

华北农学报

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类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响

温立斌¹, 何孔旺¹, 杨汉春², 郭容利¹, 钟书霖¹, 周俊明¹, 陈梦海³

1. 江苏省农业科学院兽医研究所, 农业部动物疫病诊断与免疫重点开放实验室, 国家兽用生物制品工程技术研究中心, 江苏南京210014;
2. 中国农业大学动物医学院, 北京100094;
3. 扬州大学生物科学与技术学院, 江苏扬州225009

收稿日期:2009-02-20 出版日期:2009-12-31
作者简介:作者简介:温立斌(1967-)，男，河北宣化人，博士，高级兽医师，主要从事动物分子病毒学与免疫学研究。
基金资助:
国家“973”计划前期研究专项基金(2007CB116308);江苏省自然科学基金(BK2008351)

Effects of Porcine Circovirus一like Agent Pl Infection on Counts and Proliferation Activity of T Lymphocyte in Peripheral Blood of Piglets

WENLi-bin¹, HEKong-wang¹, YANGHan-chun², GUORong-li¹, ZHONGShu-lin¹, ZHOUJun-ming¹, CHENMeng-hai³

1. Institute of Veterinary Medicine, Jiangsu Academy of Agriculture Sciences, Key Laboratory of Animal Diseases Diagnostic and Immunology, Ministry of Agriculture, National Center for Engineering Research of Veterinary Bio- products, Nanjing 210014, China;
2. College of Veterinary Medicine, China Agricultural University, Beijing 100094, China;
3. College of Biological Science and Technology, Yangzhou University, Yangzhou 225009, China

Received:2009-02-20 Published:2009-12-31

摘要/Abstract

摘要： 探讨类猪圆环病毒因子P1感染对猪细胞免疫功能的影响。将12头30日龄广西巴马小型猪随机分成试验组和对照组,每组6头。在猪感染P1后第3,8,17,24,31和40天,采用血常规和单色流式细胞术分析猪外周血T淋巴细胞的含量,通过WST法对其增殖活性进行检测。P1感染组的T淋巴细胞数量(绝对数和相对数)整体趋势低于对照组,而两组的T淋巴细胞增殖活性没有显著差别。P1感染对机体的细胞免疫功能有一定程度的影响。

关键词: 类猪圆环病毒因子, 广西巴马小型猪, T淋巴细胞计数, T淋巴细胞增殖活性

Abstract: The research is to explore the effects of porcine circovirus- like agent P1 infection on counts and prolifer ation activity of Tlymphocyte in peripheral blood of piglets. Twelve 30- day-old guangxi bama miniature pigs were ran-domly divided into two groups of six animals each. A complete haemogram and single-color flav cytometry was performed to determine the Tlymphocyte of 3, 8, 17, 24, 31 and 40 days after inoculation. meanwhile, WST assay was to do the T lymphocyte prolfferation activity. There was no significant difference in Tlymphocytes prolfferation activity between the two groups of pigs( P>0.05).On the other hand, in comparison to control pies, the means of Tlymphocyte percentages and absolute counts were overall lower in pigs infectionwith P1. Altogether, the results would suggest that P1 infection has a certain degree of influence to mount an effective cell mediated immune response.

Key words: Porcinecircovirus-likeagent, Guangxihamaminiaturepig, Tlymphocytecount, Tlymphocyteprolifer-ationactivity

中图分类号:

S852.65

WENLi-bin, HEKong-wang, YANGHan-chun, GUORong-li, ZHONGShu-lin, ZHOUJun-ming, CHENMeng-hai. Effects of Porcine Circovirus一like Agent Pl Infection on Counts and Proliferation Activity of T Lymphocyte in Peripheral Blood of Piglets[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.7668/hbnxb.2009.S1.001.

http://www.hbnxb.net/CN/Y2009/V24/IS1/1

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类圆环病毒因子P1感染对猪外周血T淋巴细胞含量和增殖活性的影响

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