摘要： 研究报道类猪圆环病毒因子P1的核酸链型和极性的研究结果。采用氯化铯平衡密度梯度离心获得较为纯净的病毒粒子后,提取病毒DNA,经S1核酸酶消化、特异性单引物第一轮PCR扩增结合常规第二轮PCR扩增等研究,结果表明,类猪圆环病毒因子P1为单股、负链基因组。
关键词:
类猪圆环病毒因子P1,
核酸链型,
极性
Abstract: The results of the nucleic acid strandedness and polarity of porcine circovirus-like agent P1 were reported here. Relatively pure viral particles were obtained by CsCl equilibrium density-gradient centrifugation. The?P1 could be determined as single strand nucleic acid and negative-strand genome by S1 nuclease treatment and two?round of PCR amplification，the first round using the single-stranded-specific primer and the second round by one?pair of specific primers.
Key words:
Porcine circovirus-like agent P1,
Nucleic acid strandedness,
Polarity
中图分类号:
温立斌, 何孔旺, 倪艳秀, 张雪寒, 李彬, 王小敏, 郭容利, 周俊明, 俞正玉, 茅爱华, 吕立新. 类猪圆环病毒因子P1核酸链型和极性的研究[J]. 华北农学报, 2012, 27(3): 120-122. doi: 10.3969/j.issn.1000-7091.2012.03.023.
WEN Li-bin, HE Kong-wang, NI Yan-xiu, ZHANG Xue-han, LI Bin, WANG Xiao-min, GUO Rong-li, ZHOU Jun-ming, YU Zheng-yu, MAO Ai-hua, LU Li-xin. Studies on the Nucleic Acid Strandedness and Polarity of Porcine Circovirus-like Agent P1[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 120-122. doi: 10.3969/j.issn.1000-7091.2012.03.023.