摘要： 为研究信号分子钙在甜菜抗丛根病过程中的作用，用三种从不同位点阻断Ca²⁺信号途径的抑制剂，即Ca²⁺螯合剂EGTA(乙二醇双四乙酸)(5 mmol/L)、Ca²⁺通道阻遏剂LaCl₃(5 mmol/L)和CaM拮抗剂W7(N-氨乙基-5-氯-1-磺胺酰萘)(0．3 mmol/L)，分别处理种植于甜菜丛根病三级病土中的甜菜，测定其抗氧化酶、O^2-、H₂O₂及可溶性蛋白质的含量，结果表明：EGTA(5 mmol/L)、LaCl₃(5 mmol/L)和W₇(0．3 mmol/L)可降低超氧化物歧化酶(SOD)、过氧化物酶(POD)、过氧化氢酶(CAT)和抗坏血酸过氧化物酶(APX)的活性，增加O^2-的积累和H₂O₂的含量，降低了可溶性蛋白质的含量。说明Ca²⁺-CaM系统与甜菜抗丛根病性相关。

关键词: Ca²⁺-CaM, 甜菜, 抗丛根病性, 抗氧化系统

Abstract: In order to study the fuction of Ca²⁺ and CaM during the resistance to rhizomania,effects of 3 inhibitors of Ca²⁺(EGTA,LaCl₃ and W₇)on antioxidant system,O^2- production rate,H₂O₂ and soluble protein content were studied in sugar beet,cultivated in rhizomania contaminated soil.The results showed that SOD activity,POD activity,CAT activity,APX activity and soluble protein content decreased while O^2- production rate and H₂O₂ content increased.It indicated a close relationship between resistance to rhizomania and Ca²⁺-CaM.

Key words: Ca²⁺-CaM, Sugar beet, Resistance to rhizomania, Physiological characteristics

中图分类号: 

• S435.663