摘要： 研究了微管解聚型三唑类化合物VHZ对玉米分生组织的影响,从细胞学及生物化学水平分析了VHZ的毒害机理.结果表明,VHZ能够诱导玉米根尖分生组织细胞前期、中期的同步化以及染色体变异(染色体桥、染色体滞后、染色体凝集).临界浓度和时间分别为20 μmol/L和12 h.免疫荧光标记检测表明,同步化细胞的形成以及染色体结构变异与纺锤丝的功能受到抑制有关.2D/SDS-PAGE检测发现,经VHZ处理后玉米根尖蛋白有两种组分消失,四种蛋白质组分含量有不同程度的降低,这些蛋白质组分可能对VHZ的防御机制有重要作用.
关键词:
VHZ,
毒害作用,
细胞分裂,
微管,
蛋白质组分
Abstract: Studied the VHZ′s toxic effect to maize meristem in cell and biochemistry level. VHZ is a microtubules depolymerization triazole compound. Experiment results revealed that prophase,metaphase synchronization division cells, chromosome structure variation (chromosome bridge,chromosome sluggish,chromosome condensation) could be induced by VHZ in root meristem of maize. The threshold concentration and time were 20μmol/ L for 12 h. Synchronization division cells and chromosome structure variation were thought to be directly related to the disturbance of spindle function by the immunofluorescence test. Two protein species were disappeared and the content of four protein species were reduced in root meristem by 2D SDS2PAGE analysis. They could play an essential role in cell defence mechanisms against VHZ.
Key words:
VHZ,
Toxic effect,
Cell division,
Microtubule,
Protein composition
中图分类号:
赵建叶, 彭永康. VHZ对玉米分生组织毒害的细胞及生化分析[J]. 华北农学报, 2009, 24(4): 235-238. doi: 10.7668/hbnxb.2009.04.048.
ZHAO Jian-ye, PENG Yong-kang. Cytological Observation and Biochemical Analysis of Maize Root Meristem Subjected to the VHZ Stress[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(4): 235-238. doi: 10.7668/hbnxb.2009.04.048.