大白菜DREB类转录因子cDNA的克隆及植物表达载体的构建

doi:10.7668/hbnxb.2009.01.016

华北农学报 ›› 2009, Vol. 24 ›› Issue (1): 69-73. doi: 10.7668/hbnxb.2009.01.016

所属专题： 白菜；

大白菜DREB类转录因子cDNA的克隆及植物表达载体的构建

李妍, 申书兴, 轩淑欣, 李晓峰, 王彦华

河北农业大学园艺学院河北保定 071001

收稿日期:2008-10-13 出版日期:2009-01-28
通讯作者: 王彦华(1970-),女,河北易县人，副教授，博士，主要从事蔬菜遗传育种与生物技术方面的研究。
作者简介:李妍(1983-)女，天津人，硕士，主要从事蔬菜遗传育种与生物技术方面的研究。
基金资助:
河北省自然科学基金(C2006000416);河北农业大学博士启动基金

Cloning the cD NA of D REB-like Transcription Factor from Chinese Cabbage and Construction of Plant Expression Vector

LI Yan, SHEN Shu-xing, XUAN Shu-xin, LI Xiao-feng, WANG Yan-hua

College of Horticulture Agricultural University of Hebei Baoding 071001 China

Received:2008-10-13 Published:2009-01-28

摘要/Abstract

摘要： 以干旱处理的大白菜自交系85-1为材料,利用RT-PCR技术获得了大白菜DREB类转录因子基因的cDNA编码序列,命名为BcDREB1(GenBank登录号为:EU924266)。序列分析表明,BcDREB1核苷酸序列长663 bp,编码214个氨基酸,含有一个典型的AP2结构域,具有DREB转录因子的典型特征。将该基因的cDNA编码序列连接到植物表达载体pCAMBIA2301中,成功构建了大白菜BcDREB1基因的植物表达载体pCAM-BcDREB1,为进一步通过转基因技术研究该基因的功能奠定了基础。

关键词: 大白菜, DREB转录因子, 基因克隆, 植物表达载体

Abstract: The cDNA of DREB transcription factor named BcDRFBl(GenBank accession numberEU924266) was ob-twined from Chinese cabbage inbred line 85-1 under the ought stress by RT-PCR method. The sequence analysis indicatedthat the clone was consisted of 663 nucleotides (nt),coding 214 amino acids. The deduced primary structure of BcDRFBlcontained a silge AP2 cbmain and showed the typical characteristics of DRFB gene family. A plant expression vector of theBcDRFBl gene was constructed and named pCAM BcDRFBl,in which the.S sequence of BcDRFBl was controlled bythe CaMV 35S promoter. This work laid the foundations for future transgenic research on BcDRFBlgene function.

Key words: Chinese cabbage, DRFB transcription factor, Cane cloning, Plant expression vector

中图分类号:

S634.1

李妍, 申书兴, 轩淑欣, 李晓峰, 王彦华. 大白菜DREB类转录因子cDNA的克隆及植物表达载体的构建[J]. 华北农学报, 2009, 24(1): 69-73. doi: 10.7668/hbnxb.2009.01.016.

LI Yan, SHEN Shu-xing, XUAN Shu-xin, LI Xiao-feng, WANG Yan-hua. Cloning the cD NA of D REB-like Transcription Factor from Chinese Cabbage and Construction of Plant Expression Vector[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2009, 24(1): 69-73. doi: 10.7668/hbnxb.2009.01.016.

http://www.hbnxb.net/CN/Y2009/V24/I1/69

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大白菜DREB类转录因子cDNA的克隆及植物表达载体的构建

Cloning the cD NA of D REB-like Transcription Factor from Chinese Cabbage and Construction of Plant Expression Vector

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