摘要： 通过RAPD技术对桃、李、杏、梅、樱类植物及桃种内的不同类群的DNA扩增带的多态信息量的统计分析,结果表明:供试的21个引物均能为桃类植物提供较大的多态信息量,樱类和梅类的多态信息位于其次,而李类和杏类则较低。有11个引物最适用于桃、李、杏、梅、樱类的群体样品的多态性检测,每类植物均有各自能提供较大信息量的引物,其变化由李类只有1个引物到桃类有21个引物。在桃种内的各类群中,供试引物在黄肉桃、红叶桃、蟠桃、蜜桃类群中的信息量较大,有9个引物较适宜于对桃种内的各类群多态信息量分析;对于单个类群提供最大信息量的引物,从水蜜桃只有1个引物到黄肉桃有18个。根据多态信息量的指标,为桃及其李属近缘种植物的分子生物学分析,提供直接可供选择的引物和比较直观确定供试样品的遗传多样性。

关键词: 李属内种类, 桃种内类群, 多态信息量

Abstract: Polymorphism information contents (PIC) of amplified bands among different groups in Prunus persica and related species in Prunus were analyzed using RAPD(Randomly Amplif ied Polymorphism DNA) technology. The results expressed: The experimented 21 primers could provided the highest PIC in Peach and related species in Prunus as thefirst, cherry ( P. cerasus, P. avium) and mume(P. mume) as the second, plum(P. salicina ) and apricot(P. armeniaca) as the third. 11 primers were suitable for detecting PIC of accessions composed of above mentioned species.Each species had its primers with higher PIC whose value changed from 1 for P. salicina to 21 for P.persica. The experimented primers could provided higher PIC in Yellow peach, Red??leaf peach, Flat peach, Juicy peach among groups. 9primers were considerable for providing higher PIC for accessions among different groups in Prunus persica. Primers that could provide higher PIC in single group changed from 1 for Juicy peach to 18 for Yellow peach. According to indexes of PIC, they could be used in molecular analysis in prunus, proving selectable primers and diversity for the experiment accessions direct ly perceived through the senses.

Key words: Species in prunus, Groups in Prunus persica, Polymorphism information contents (PIC)

中图分类号: 

• S662