蒙古冰草<em>Lea3</em>抗旱基因片段的分离与鉴定

doi:10.7668/hbnxb.2008.06.014

华北农学报 ›› 2008, Vol. 23 ›› Issue (6): 64-67. doi: 10.7668/hbnxb.2008.06.014

所属专题： 抗旱节水；生物技术；

蒙古冰草Lea3抗旱基因片段的分离与鉴定

赵彦^1,2, 云锦凤¹, 石凤敏¹, 高翠萍¹

1. 内蒙古农业大学, 生态环境学院, 内蒙古, 呼和浩特, 010018;
2. 蒙古农牧业科学院, 内蒙古, 呼和浩特, 010031

收稿日期:2008-06-10 出版日期:2008-12-28
通讯作者: 云锦凤(1941－)，女，内蒙古呼和浩特人，教授，博士生导师，主要从事牧草遗传育种研究。
作者简介:赵彦(1977－)，女，内蒙古通辽人，助理研究员，在读博士，主要从事植物分子遗传学研究。
基金资助:
国家自然科学基金资助项目(30760159)；国家“973”项目子课题(2007CB108901-1)

Isolation and Characterization of a Lea3 Gene Fragment from Agropyron mongolicum Keng

ZHAO Yan^1,2, YUN Jin-feng¹, SHI Feng-min¹, GAO Cui-ping¹

1. College of Entironment, Inner Mongolia Agricultural University, Huhhot 010018, China;
2. Inner Mongolia Academy of Agriculture and Animal Husbandry Sciences, Huhhot 010031, China

Received:2008-06-10 Published:2008-12-28

摘要/Abstract

摘要： 研究旨在利用同源序列法分离蒙古冰草抗旱基因同源片段。根据已知禾本科植物抗旱基因Lea第3组的保守区段设计一对简并引物，采用PCR方法对蒙古冰草幼苗的基因组DNA进行扩增。获得了1个蒙古冰草的抗旱基因Lea3基因片段，长度为97 bp，包含12 bp的非翻译区，共编码12个氨基酸。核苷酸序列分析表明，该序列与小麦抗旱基因LEA第3组的Wrab19同源性为93%，与小麦受ABA诱导的WRAB1基因同源性为93%；与一个来源于玉米mRNA的逆境胁迫基因克隆912同源性为96%；与大麦受ABA诱导的pHVA1基因同源性为9%；与大麦HVA1基因同源性为91%。Southern杂交表明该基因在蒙古冰草基因组中以基因家族形式存在。

关键词: 蒙古冰草, Lea基因, 同源序列

Abstract: In this study,drought resistance gene homology fragment fromAgropyron mongolicumKeng was isolated using homology-based method.A pair of degeneracy primers was designed according to the amino acid conserved regions of the clonedGramineaeplant group 3Leagenes,One drought resistance gene has been obtained.by the polymerase chain reaction(PCR)using the genomic DNA of A.mongolicumKeng as template.It was named asLea3.The nucleotide sequences was 497 bp,including 124 bp untranslated region,and encoded 124 amino acids.Homology research showed that the nucleotides ofLea3 was 93 % identical to wheat group 3Leagene Wrab19,93 % identical to wheat gene WRAB1,96 % identical to the clone 9124 from the mRNA of maize,94 % identical to barley genepHVA1,and 91 % identical to barley geneHVA1.the analysis of its secondary structure and hydrophilicity indicated that 2-helixes were predominant feature of secondary structure,and this gene had high hydrophilic property.The result of Southern blot analysis revealed the existence of anLea3 gene family in the A.mongolicum Keng genome.

Key words: Agropyron mongolicumKeng, Leagene, Homology Sequence

中图分类号:

S543

赵彦, 云锦凤, 石凤敏, 高翠萍. 蒙古冰草Lea3抗旱基因片段的分离与鉴定[J]. 华北农学报, 2008, 23(6): 64-67. doi: 10.7668/hbnxb.2008.06.014.

ZHAO Yan, YUN Jin-feng, SHI Feng-min, GAO Cui-ping. Isolation and Characterization of a Lea3 Gene Fragment from Agropyron mongolicum Keng[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2008, 23(6): 64-67. doi: 10.7668/hbnxb.2008.06.014.

http://www.hbnxb.net/CN/Y2008/V23/I6/64

参考文献

[1] 俞嘉宁,山仑.Lea蛋白与植物的抗旱性[J].生物工程进展,2002,22(2):10-14.
[2] 刘娥娥,汪沛洪,郭振飞.植物的抗旱诱导蛋白[J].植物生理学通讯,2001,37(2):155-160.
[3] 张永恩,李潮海,王群.植物抗旱相关基因研究进展[J].中国农学通报,2004,20(6):85-88.
[4] 孙立平,李德全.Lea蛋白的分子生物学研究进展[J].生物技术通报,2003(6):5-13.
[5] 马媛媛.抗旱基因Lea的克隆及其序列解析[D].哈尔滨:东北林业大学,2003.
[6] Dare L,Greanway S C,Galau G A.Developmental biochemistry of cotton seed embryogenessis and germination:changing mRNA population as shown in vitro and in vivo protein synthesis[J].Biochemistry,1981,20:4162-4168.
[7] Dure L,Crouch M,Harada J,et al.Common amino acid sequence domains among the Lea proteins of higher plants[J].Plant Mol Biol,1989,12:475-486.
[8] Dure L.A repeating 11-mers amino acid motif and plant desiccation[J].Plant J,1993,3:363-369.
[9] Hsing Y C,Chen Z Y,Shin M D,et al.Unusual sequence of groups 3 Lea mRNA inducibleby maturation or drying in soybean seeds[J].Plant Mol Biol,1995,29:863-868.
[10] Baker.Sequence and characterization of 6 Lea proteins and their genes from cotton[J].Plant Mo1 Biol,1988,11:277-291.
[11] Ingram J,Bartels D.The molecular basis of dehydration tolerance in plant[J].Annu Rev Plant Physiol Plant Not Biol,1996,47:377-403.
[12] Ried J L,Walker-Simmons M K.Group 3 late embryo enesis abundant proteins in desiccation tolerant seedlings of wheat(Trit.icum aestivum)[J].Plant Physiol,1993,102:125-131.
[13] Chandler P M,Robertson M.Gene expression regulated by abscisic acid and its relation to stress tolerance[J].Annu Rev Plant physiol plant Mol Bio,1994,45:113-141.
[14] 赖钟雄,张妙霞,李冬梅.植物Lea蛋白与Lea基因表达[J].福建农林大学学报:自然科学版,2002,31(4):463-466.

选择文件类型/文献管理软件名称

选择包含的内容

蒙古冰草Lea3抗旱基因片段的分离与鉴定

Isolation and Characterization of a Lea3 Gene Fragment from Agropyron mongolicum Keng

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价

[1]	却志群, 沈春修, 卢其能. 东乡野生稻NBS类抗病基因同源片段的克隆及序列分析[J]. 华北农学报, 2011, 26(1): 78-82.
[2]	石凤敏, 云锦凤, 赵彦, 张瑞霞. 蒙古冰草基因组类反转录转座子基因同源序列的克隆与序列分析[J]. 华北农学报, 2010, 25(6): 52-56.
[3]	张楠, 王海燕, 刘大群. 小麦STK类抗病基因同源序列的克隆与分析[J]. 华北农学报, 2010, 25(5): 20-24.
[4]	张众, 云锦凤, 逯晓萍, 温超, 贾利霞. 氮磷钾多元复合肥对蒙古冰草种子生产效应研究[J]. 华北农学报, 2007, 22(S3): 41-44.
[5]	吕香玲, 卢峰, 宋波. 利用同源序列发掘抗病候选基因的方法[J]. 华北农学报, 2007, 22(S2): 10-12.
[6]	张众, 云锦凤, 逯晓萍, 王润莲, 温超, 贾利霞. 不同播种因素对蒙古冰草种子产量和品质的影响[J]. 华北农学报, 2006, 21(6): 95-99.

模态框（Modal）标题

选择文件类型/文献管理软件名称

选择包含的内容

蒙古冰草Lea3抗旱基因片段的分离与鉴定

Isolation and Characterization of a Lea3 Gene Fragment from Agropyron mongolicum Keng

PDF

可视化

被引次数

摘要/Abstract

引用本文

使用本文

参考文献

相关文章 6

编辑推荐

Metrics

本文评价