华北农学报 ›› 2007, Vol. 22 ›› Issue (S2): 257-259. doi: 10.7668/hbnxb.2007.S2.061

所属专题: 棉花

• 论文 • 上一篇    下一篇

四种棉花黄萎病毒素制备方法的比较

马春红1, 范尉尉2, 董文琦3, 刘子会1, 贾银锁1   

  1. 1. 河北省农林科学院遗传生理研究所, 河北 石家庄 050051;
    2. 石家庄市疾病预防控制中心, 河北 石家庄 050011;
    3. 河北省农林科学院, 河北 石家庄 050051
  • 收稿日期:2007-03-20 出版日期:2007-12-31
  • 通讯作者: 贾银锁(1950-),男,河北新乐人,博士,研究员,主要从事作物生理及遗传育种的研究工作.
  • 作者简介:马春红(1968-),女,浙江金华人,副研究员,主要从事作物抗病生理及遗传育种研究
  • 基金资助:
    河北省自然科学基金项目资助(C2006000744);国际科技合作资助项目(2006DFB02480)

A Comparison of Verticillium dahliac Kleb Determined in Cotton by Four Extracting Methods

MA Chun-hong1, FAN Wei-wei2, DONG Wen-qi3, LIU Zi-hui1, JIA Yin-suo1   

  1. 1. Institute of Genetics and Physiology,Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China;
    2. Shijiazhuang Center for Disease Control and Prevention,Shijiazhuang050011,China;
    3. Hebei Academy of Agriculture and Forestry Sciences,Shijiazhuang 050051,China
  • Received:2007-03-20 Published:2007-12-31

摘要: 多年来大丽轮枝菌毒素的制备过程中常用几种不同的制备方法,究竟不同方法制备的毒素在毒蛋白含量上与致病力上有多大区别,长期以来一直没有定量分析的相关报道。本文就几种常见的方法加以比较,在相同条件下本试验采用的方法四为最佳的试验方法。

关键词: 棉花, 黄萎病菌, 毒素, 制备方法, 比较

Abstract: Verticillium dahlias Kleb toxin extraction has been commonly used several extracting methods for many years.Actually,the different methods extracting toxin is a significant difference in the poisonous protein content and pathogencity.It has not reported the correlative quantitative analysis for a long-time about it.The comparison of the article with several four common extracting methods was test and selected the best one in the experiment.

Key words: Cotton, Verticillium dahliae Kleb, Toxin, Extracting method, Comparison

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引用本文

马春红, 范尉尉, 董文琦, 刘子会, 贾银锁. 四种棉花黄萎病毒素制备方法的比较[J]. 华北农学报, 2007, 22(S2): 257-259. doi: 10.7668/hbnxb.2007.S2.061.

MA Chun-hong, FAN Wei-wei, DONG Wen-qi, LIU Zi-hui, JIA Yin-suo. A Comparison of Verticillium dahliac Kleb Determined in Cotton by Four Extracting Methods[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(S2): 257-259. doi: 10.7668/hbnxb.2007.S2.061.

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