摘要： 采用醇溶蛋白电泳法对进口香米的品种纯度进行鉴定,首先需要构建一个进口香米品种的标准图谱来做为品种鉴定的基准。本试验收集了宁波口岸进口的香米、大米以及白糯米3种样品,用SDS-PAGE(十二烷基硫酸钠—聚丙烯酰胺凝胶电泳)鉴定方法开展了具体的试验工作。通过对香米、大米以及白糯米3种样品的筛选与电泳分析,确定了上述样品醇溶蛋白的电泳图谱,并在此基础上构建了各样品醇溶蛋白在B,C两组区间的电泳图谱。同时,本试验还对影响电泳图谱的若干因素进行了研究和探索,为今后具体香米品种鉴定工作的开展提供了一定的试验依据。
关键词:
香米纯度,
电泳图谱,
鉴定方法
Abstract: To identify the purity of import Thai Hom Mali Rice by the method of electrophoresis,we need to build a standard electrophoretogram of import rice as a benchmark.So we collected three kinds of import rice which are Thai Hom Mali Rice,normal rice and sticky rice from Ningbo Port in recent years.Then,we gained some electrophoresis pattern of the prolamine which extracted from these three kinds of samples by SDS-PAGE(sodium dodecyl sulfate-polyacrylamide gel electrophoresis).Based on it,we worked out the three samples′electrophoresis pattern of prolamine between B and C section.In addition,we also investigated some factors which can probably affect the result of electrophoresis pattern and all of these will provide some reference for the future experiments of the identification of the purity of the import Thai Hom Mali Rice.
Key words:
The purity of Thai Hom Mali Rice,
SDS- PAGE,
Identification
中图分类号:
荣德福, 徐颖, 何阳, 李翠凤, 周强. SDS-PAGE电泳法鉴定进口香米纯度方法的研究[J]. 华北农学报, 2007, 22(S2): 81-84. doi: 10.7668/hbnxb.2007.S2.021.
RONG De-fu, XU Ying, HE Yang, LI Cui-feng, ZHOU Qiang. The Research of Identification the Purity of Thai Hom Mali Rice by SDS-PAGE[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(S2): 81-84. doi: 10.7668/hbnxb.2007.S2.021.