大豆中2个编码PR10蛋白新基因的克隆与分析

doi:10.3969/j.issn.1000-7091.2013.04.002

摘要/Abstract

摘要： 从抗大豆花叶病毒病(SMV)大豆材料科丰1号中克隆到2个编码PR10蛋白的新基因Glyma09g04530和Glyma15g15590,Glyma09g04530和Glyma15g15590基因的ORF全长均为477 bp,编码158个氨基酸。序列比对与系统发生树分析结果表明:Glyma09g04530和Glyma15g15590是大豆中2个编码PR10蛋白的新基因。Glyma09g04530和Glyma15g15590基因在大豆的根、茎及叶中均有表达;接种大豆SMV后,Glyma09g04530和Glyma15g15590基因在大豆科丰1号的叶片中的表达均被强烈诱导并高效表达,显示Glyma09g04530和Glyma15g15590基因与大豆SMV抗性密切相关。

关键词: 大豆, PR10蛋白, 基因克隆, 表达分析

Abstract: Two new soybean genes named Glyma09g04530 and Glyma15g15590 which encoding PR10 proteins were cloned from a SMV resistant cultivar Kefeng No． 1． Glyma09g04530 and Glyma15g15590 have a 477 bp ORF and coding 158 amino acids． Sequence alignment and evolutionary tree clustering analysis showed that Gly-ma09g04530 and Glyma15g15590 are new PR10 protein genes in soybean． The genes of Glyma09g04530 and Gly-ma15g15590 expressed in root， stem and leave by using Real- time PCR analysis in soybean． Expression level of Gly-ma09g04530 and Glyma15g15590 were strongly induced and highly expressed in leaves of Kefeng No． 1 after inocu-lation SMV． We speculate that Glyma09g04530 and Glyma15g15590 may be involved for getting resistance to SMV．Key words: Soybean; PR10 protien; Gene cloning; Expression analysis

Key words: Soybean, PR10 protien, Gene cloning, Expression analysis

中图分类号:

陈华涛, 陈新, 顾和平, 张红梅, 袁星星, 崔晓艳. 大豆中2个编码PR10蛋白新基因的克隆与分析[J]. 华北农学报, 2013, 28(4): 7-12. doi: 10.3969/j.issn.1000-7091.2013.04.002.

CHEN Hua-tao, CHEN Xin, GU He-ping, ZHANG Hong-mei, YUAN Xing-xing, CUI Xiao-yan. Cloning and Analysis of Two New PR10 Members in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(4): 7-12. doi: 10.3969/j.issn.1000-7091.2013.04.002.

http://www.hbnxb.net/CN/Y2013/V28/I4/7

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