百脉根S-腺苷甲硫氨酸脱羧酶基因克隆与表达分析

doi:10.3969/j.issn.1000-7091.2013.02.015

华北农学报 ›› 2013, Vol. 28 ›› Issue (2): 78-85. doi: 10.3969/j.issn.1000-7091.2013.02.015

所属专题： 生物技术；

百脉根S-腺苷甲硫氨酸脱羧酶基因克隆与表达分析

路玉兰, 孙艳香, 冯雪, 赵学良

廊坊师范学院生命科学学院遗传与育种研究所, 河北廊坊 065000

收稿日期:2013-01-19 出版日期:2013-04-28
通讯作者: 赵学良(1961－)，男，河北廊坊人，教授，硕士，主要从事植物生理学与细胞工程教学和研究工作。
作者简介:路玉兰(1965－)，女，河北廊坊人，副教授，硕士，主要从事生物学教学和研究工作。
基金资助:
廊坊师范学院博士专项基金资助项目(LSZB200903)

Cloning and Expression Analysis of S-adenosylmethionine Decarboxylasegene from Lotus corniculatus L．

LU Yu-lan, SUN Yan-xiang, FENG Xue, ZHAO Xue-liang

Institute ofgenetics and Breeding, Langfang Teachers College, Langfang 065000, China

Received:2013-01-19 Published:2013-04-28

摘要/Abstract

摘要： S-腺苷甲硫氨酸脱羧酶(SAMDC|EC 4.1.1.50)是多胺合成过程中的限速酶。以日本百脉根EST序列为基础,通过设计特异引物进行RT-PCR扩增,获得了S-腺苷甲硫氨酸脱羧酶编码cDNA,命名为LcSAMDC1。LcSAMDC1基因组序列全长2 626 bp,存在3个内含子|cDNA序列全长1 799 bp,存在3个植物SAMDC基因特征性ORF,其中tORF编码2个氨基酸残基,sORF编码55个氨基酸残基,mORF编码354个氨基酸残基|mORF编码产物推断的分子量为38.6 kDa,具有2个高度保守的功能结构域(酶原剪切位点和PEST结构域)|半定量RT-PCR分析显示,LcSAM-DC1在幼叶和根中具有高水平的表达,在茎中表达水平较低,而在成熟叶片中不表达。

关键词: 百脉根, 多胺, S-腺苷甲硫氨酸脱羧酶, LcSAMDC1

Abstract: S-adenosylmethionine decarboxylase(SAMDC;EC 4. 1. 1. 50)is a key rate-limiting enzyme located in the polyamine biosynthesis pathway. According to the sequence information of EST from Lotus corniculatus var. ja-ponicus Regel,a cDNA of SAMDC was cloned from Lotus corniculatus,and named LcSAMDC1. The full-lengthge-nome sequence of LcSAMDC1 was 2 626 bp,including 3 introns. The full-length cDNA sequence of LcSAMDC1 was 1 799 bp,with 3 characteristic ORFs,which were tORF,sORF and mORF,coding 2,55,354 amino acid residues re-spectively. The deducted protein of mORF was with 38. 6 kDa molecular weight,and had two highly conserved function domains(proenzyme cleavage site and PEST domain). Semi-quantitative RT-PCR analysis showed that,the expression of LcSAMDC1 was higher in young leaves and roots than in stems,but failed to be detected in mature leaves.

Key words: Lotus corniculatus L． , Polyamine, S-adenosylmethionine decarboxylase, LcSAMDC1

中图分类号:

路玉兰, 孙艳香, 冯雪, 赵学良. 百脉根S-腺苷甲硫氨酸脱羧酶基因克隆与表达分析[J]. 华北农学报, 2013, 28(2): 78-85. doi: 10.3969/j.issn.1000-7091.2013.02.015.

LU Yu-lan, SUN Yan-xiang, FENG Xue, ZHAO Xue-liang. Cloning and Expression Analysis of S-adenosylmethionine Decarboxylasegene from Lotus corniculatus L．[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(2): 78-85. doi: 10.3969/j.issn.1000-7091.2013.02.015.

http://www.hbnxb.net/CN/Y2013/V28/I2/78

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