华北农学报

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不同环境条件对球孢白僵菌HFW-05的敏感性变化

宋健, 曹伟平, 陆晴, 杜立新   

  1. 河北省农林科学院植物保护研究所河北省农业有害生物综合防治工程技术研究中心, 河北 保定 071000
  • 收稿日期:2012-07-29 出版日期:2012-12-31
  • 通讯作者: 杜立新(1978 - ), 男, 河北武安人, 助理研究员, 博士, 主要从事生物防治研究。
  • 作者简介:宋 健(1980 - ), 女, 河北涿州人, 助理研究员, 硕士, 主要从事杀虫微生物研究。
  • 基金资助:
    河北省财政项目(F12C10034);国家“948”项目(2011-G4);国家“863”项目(2011AA10A201)

Sensitivity to Changes of Environmental Factors on Beauveria bassiana HFW-05 Strain

SONG Jian, CAO Wei-ping, LU Qing, DU Li-xin   

  1. Institute of Plant Protection,Hebei Academy of Agriculture and Forestry Sciences, IPM Centre of Hebei Province,Baoding 071000,China
  • Received:2012-07-29 Published:2012-12-31

摘要: 通过对温度和紫外线等条件的变化,明确了该因素对HFW-05菌株生长与毒力的影响。结果表明,25℃培养有利于提高孢子萌发率及菌株产孢量;30℃培养有利于提高菌株产酶量,最适菌株生长的温度范围是25~30℃。生测试验表明,HFW-05菌株对小菜蛾的最佳致死温度范围为25~30℃。菌株在25℃下经紫外线1 h处理后,萌发率可比对照增加12%,产孢量增加26%,表明适量紫外处理有利于菌株生长。

关键词: 球孢白僵菌, 环境, 胞外蛋白酶活性, 毒力

Abstract: To explore sensitivity changes of HFW-05 strain treated by different temperature and ultraviolet irradiation, the growth and toxicity of the strain under different temperature and UV radiation was studied. Results indicated that germination and sporulation of the strain was appropriate at 25℃ , and the enzyme production could increase at 30℃ . The growth rate and sporulation were increased at 35℃ with UV radiation under 1 h, but the growthrate would reduce with postponement of the exposure time. The lethal rate of the Plutella xylostella ( Linnaeus) was93 . 1 % by the treatment of 30℃ , and it could effectively control the population of insect.

Key words: Beauveria bassiana, Entironment, Activity of extracellular protease, Toxicity

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引用本文

SONG Jian, CAO Wei-ping, LU Qing, DU Li-xin. Sensitivity to Changes of Environmental Factors on Beauveria bassiana HFW-05 Strain[J]. ACTA AGRICULTURAE BOREALI-SINICA, doi: 10.3969/j.issn.1000-7091.2012.z1.070.

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