花生蔗糖合成酶基因的克隆及序列分析

doi:10.3969/j.issn.1000-7091.2012.04.005

摘要/Abstract

摘要： 蔗糖合成酶（Sucrose Synthase，SuSy）是蔗糖代谢途径中的关键酶，在植物生长、发育和渗透调节过程中起着重要作用。为揭示蔗糖合成酶基因在花生中的抗逆机理，以花生基因组DNA为模板，利用染色体步移技术（Genome Walking）中的TAIL-PCR技术扩增花生SuSy基因组序列和启动子区域，得到基因组序列6189bp，启动子预测分析表明，该序列包含约800bp的启动子上游调控序列，13个外显子，12个内含子。启动子元件分析显示，该片段含有典型的TATA-box、CAAT-box，并存在低温响应元件LTR、GA响应元件、干旱响应MYB结合位点、厌氧诱导必要的顺式作用元件ARE等调控元件，及G-box、box4等光响应元件，说明花生SuSy基因的表达可能受低温、干旱、缺氧、光照等环境因素以及激素GA的调控。

关键词: 花生, 蔗糖合成酶, 基因克隆, 启动子, 染色体步移

Abstract: Sucrose Synthase （SuSy）,a key enzyme in sucrose metabolism, plays very role in both plant growth and developmental process. It is reported that SuSy gene is also closely relate to the ability of plant resistance to abiosmotic stress. In order to reveal the mechanism of SuSy gene in peanut, here, the TAIL-PCR technology of genome walking was used to obtain a genome sequence for the length of 6 189 bp,which included 13 exons, 12 introns and about an 825 bp length partial promoter region. Sequence analysis showed that typical elements as TATA-box, CAAT-box and some other regulate elements were contained in this region, such as cis-acting element involved in low temperature responsive element （LTR）, GA responsive element,drought responsive of MYB hinging site, anae-robic induction element ARE and light responsive element G-box and box4 were found in the sequence. It was sup- posed that the expression of SuSy gene was induced by low temperature,drought,anaerobic and hormone GA etc.

Key words: Peanut, SuSy gene, Gene cloning, Promoter, Genome walking

中图分类号:

何美敬, 刘立峰, 穆国俊, 侯名语, 陈焕英, 崔顺立. 花生蔗糖合成酶基因的克隆及序列分析[J]. 华北农学报, 2012, 27(4): 23-28. doi: 10.3969/j.issn.1000-7091.2012.04.005.

HE Mei-jing, LIU Li-feng, MU Guo-jun, HOU Ming-yu, CHEN Huan-ying, CUI Shun-li. Gene Cloning and Sequence Analysis of SuSy Gene in Peanut （ Arachis hypogaea L. ）[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(4): 23-28. doi: 10.3969/j.issn.1000-7091.2012.04.005.

http://www.hbnxb.net/CN/Y2012/V27/I4/23

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