大豆<em>GmHKT6;2</em>基因的克隆与表达特性分析

doi:10.3969/j.issn.1000-7091.2012.03.001

华北农学报 ›› 2012, Vol. 27 ›› Issue (3): 1-5. doi: 10.3969/j.issn.1000-7091.2012.03.001

所属专题： 油料作物；生物技术；

• 论文 • 下一篇

大豆GmHKT6;2基因的克隆与表达特性分析

陈华涛¹, 陈新¹, 顾和平¹, 陈满峰², 张红梅¹, 袁星星¹, 崔晓艳¹

1. 江苏省农业科学院蔬菜研究所, 江苏南京 210014;
2. 江苏沿江地区农科所, 江苏如皋 22654

收稿日期:2012-02-16 出版日期:2012-06-28
通讯作者: 陈新(1970-), 男, 江苏射阳人, 研究员, 博士, 主要从事豆类作物遗传育种研
作者简介:陈华涛（1980-）, 男, 河南周口人, 助理研究员, 博士, 主要从事大豆耐逆分子生物学研究。
基金资助:
转基因生物新品种培育重大专项(2011ZX08004-004-004);江苏省农业科技自主创新项目(cx(10)409cx(10)459)

Cloning and Expression Pattern Analysis of GmHKT6 ;2 in Soybean

CHEN Hua-tao¹, CHEN Xin¹, GU He-ping¹, CHEN Man-feng², ZHANG Hong-mei¹, YUAN Xing-xing¹, CUI Xiao-yan¹

1. Institute of Vegetable Crops, Jiangsu Academy of Agricultural Sciences, Nanjing 210014, China;
2. Institute of Agricultural Sciences of the Area Along Yangtse of Jiangsu, Rugao 226541, China

Received:2012-02-16 Published:2012-06-28

摘要/Abstract

摘要： 为探明大豆中HKT蛋白基因的耐盐作用机理,从耐盐大豆材料中克隆到GmHKT6;2基因完整的cDNA序列,GmHKT6;2基因的开放阅读框(ORF)全长1 644 bp,编码547个氨基酸。序列比对与进化树分析表明:GmHKT6;2是大豆中的一个新HKT蛋白基因;GmHKT6;2基因在大豆的根、茎及叶中均能表达,150 mmol/L NaCl处理后,该基因在大豆根、茎及叶中的表达被强烈诱导并高效表达。结构域分析结果表明:大豆GmHKT6;2基因拥有10个可能的跨膜结构域(TMD)和阳离子转运蛋白保守结构域,推测其是通过调节相关阳离子的转运来调控大豆的耐盐性。

关键词: 大豆, 耐盐性, 基因克隆, 表达分析

Abstract: In order to investigate the salt tolerance mechanism of the HKT protein genes in soybean，a novel soybean gene GmHKT6;2 was identified in a salt tolerant soybean cultivar. GmHKT6;2 containing an open reading frame( ORF) of 1 644 nucleotides. The ORF encoded a protein of 547 amino acids. Sequence alignment and evolutionary tree clustering analysis showed that GmHKT6;2 is a new HKT protein gene in soybean. GmHKT6;2 gene were expressed in root，stem and leaves by using Real-Time PCR analysis in soybean. GmHKT6;2 was strongly induced and highly expressed in root，stem and leaves after treatment by 150 mmol/L NaCI. Domain analysis showed that:the soybean GmHKT6;2 gene with 10 putative transmembrane domain(TMD)and cation transport protein conserved domain，suggesting that GmHKT6;2 gene may be through control canon transporter to regulate the salt tolerance in soybean.

Key words: Soybean, Salt tolerance, Gene cloning, Expression analysis

中图分类号:

S565.1

陈华涛, 陈新, 顾和平, 陈满峰, 张红梅, 袁星星, 崔晓艳. 大豆GmHKT6;2基因的克隆与表达特性分析[J]. 华北农学报, 2012, 27(3): 1-5. doi: 10.3969/j.issn.1000-7091.2012.03.001.

CHEN Hua-tao, CHEN Xin, GU He-ping, CHEN Man-feng, ZHANG Hong-mei, YUAN Xing-xing, CUI Xiao-yan. Cloning and Expression Pattern Analysis of GmHKT6 ;2 in Soybean[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2012, 27(3): 1-5. doi: 10.3969/j.issn.1000-7091.2012.03.001.

http://www.hbnxb.net/CN/Y2012/V27/I3/1

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大豆GmHKT6;2基因的克隆与表达特性分析

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