摘要： 利用利迪链霉菌生防菌株A02发酵液和番茄灰霉病菌对番茄植株进行诱导和接种处理，利用分光光度法测定处理前和处理后1～5 d番茄叶片组织的主要防御酶系——苯丙氨酸解氨酶、过氧化物酶、过氧化氢酶和多酚氧化酶的活性变化。试验结果显示，不接种灰霉病菌而单独诱导处理、接种灰霉病菌后诱导处理和诱导处理后接种灰霉病菌这3种处理都能提高番茄植株各防御酶系的活性，后者酶活性峰值最高，对番茄植株灰霉病的抑制作用也最明显，控制效果达94.92%。
关键词:
利迪链霉菌A02,
诱导抗病性,
番茄灰霉病,
防御酶系
Abstract: The study on inducing resistant effect of Streptomyces lydicus A02 to tomato gray mold were carried out in greenhouse. Tomato seedlings were treated via three methods: only inducing with fermentation broth of Streptomyces lydicus A02, inducing after inoculating with spore suspension of Botrytis cinerea, inoculating after inducing. The activities of 4 defense enzymes in tomato leaves were assayed by ultraviolet-visible absorption spectroscopy. The results showed that all the three treatments could increase the activities of defense enzymes, of which the treatment of inoculating after inducing presented a better inducing effect on 4 defense enzymes and a better control effect on tomato gray mold than inducing after inoculating did. The control effect was up to 94.92%.
Key words:
Streptomyces lydicus A02,
Induced resistance,
Botrytis cinerea,
Defense enzymes
中图分类号:
刘宇, 刘建华, 刘伟成, 裘季燕, 刘德文, 段玉玺. 利迪链霉菌A02诱导番茄抗灰霉病作用机制研究——对植株防御酶系的影响[J]. 华北农学报, 2007, 22(2): 152-155. doi: 10.3321/j.issn:1000-7091.2007.02.037.
LIU Yu, LIU Jian-hua, LIU Wei-cheng, QIU Ji-yan, LIU De-wen, DUAN Yu-xi. The Inducing Effect of Streptomyces lydicus A02 on Tomato Resistance to Botrytis cinerea-Impact on Defense Enzymes of Tomato Seedlings[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(2): 152-155. doi: 10.3321/j.issn:1000-7091.2007.02.037.