摘要： 以富有柿果为材料，用RNA提取试剂盒提取总RNA，根据已报道的柿果ACC氧化酶(1-aminocyclopropane-l-carboxylic acid oxidase，ACO)基因的序列设计并合成1对引物，通过RT-PCR方法获得1条约1.2 kb特异片段，把该片段连接到pGEM-T easy vector上进行测序，其全长共1237 bp，编码区697 bp，共编码231个氨基酸残基。序列分析结果表明，该序列与DK-ACO1的cDNA序列同源性达99%，氨基酸的同源性达98%。

关键词: 柿果, ACC氧化酶, RT-PCR, 序列分析

Abstract: Total RNA was extracted from Fuyu persimmon fruits and reverse transcribed to first strand of cDNA. A pair of primers were designed and synthesized based on the sequence reported. The specific PCR product of 1-aminocyclopropane-1-carboxylic acid oxidase was obtained using the first strand of cDNA as template. The product was ligased to pGEM-T easy vector and sequenced. The sequencing data showed that the PCR product was 1237 bp with 697 bp of coding region, encoding 231 predicted amino acid residues. Comparison with the cDNA sequence from DK-ACO1 indicated the homology was 99%, and amino acid identities were 98%.

Key words: Persimmon fruit, Fuyu, ACC oxidase, RT-PCR, Sequence analysis

中图分类号: 

• S665.2