摘要： 测定分析了叶甲科（Chrysomelidae）15种昆虫mtDNA_Cyt b基因部分序列，结果显示，在获得的390 bp序列中，198个核苷酸位点为多态性位点（约占50.8%），序列间碱基差异平均值为18.9%，A+T含量为72.7%，碱基转换呈现明显的TC偏向性，颠换主要以TA为主，转换/颠换（R）值为0.8。依据分子数据建立了该15种昆虫的系统发育关系，结果表明：跳甲亚科（Alticinae）和叶甲亚科（Chrysomelinae）的亲缘关系较近，萤叶甲亚科（Galerucinae）与前两者的关系较远。萤叶甲亚科中瓢萤叶甲为最早分化的类群，长跗萤叶甲属（Monolepta）、凹翅萤叶甲属（Paleosepharia）、长刺萤叶甲属（Atrachya）能形成姊妹群。克萤叶甲属（Cneorane）和守瓜属（Aulacophora）的亲缘关系较近。基于蛋白质序列构建的系统树的置信值高于核苷酸序列建立的系统发育树。

关键词: 鞘翅目, 叶甲科, mtDNA-Cyt b, 系统发育关系

Abstract: Parts of mtDAN-Cyt b gene were sequenced and analyzed in 15 species of Chrysomelidae.The obtained sequence data revealed the considerable variation in 198 nucleotide sites(about 50.8%).The average difference between sequences was 18.9% and A+T was about 72.7%.Transition and transversion represented the base of TC and TA respectively.The ratio of TS/TV(R) was 0.8.The constructed phylogenetic trees showed that Alticinae has closer relationship with Chrysomelinae,compared with Galerucinae;Oides was the earliest-differentiated population.The relationship of Monolepta,Paleosepharia and Atrachya was close;Cneorane and Aulacophora could form a sister group.Phylogenetic trees based on protein sequences were more reliable than those based on nucleotide data.

Key words: Coleoptera, Chrysomelidae, mtDAN_Cyt b, Phylogenetic analysis

中图分类号: 

• Q969