原生质体非对称融合获得花椰菜与OgucMS甘蓝型油菜种间杂种

doi:10.3321/j.issn:1000-7091.2006.03.016

华北农学报 ›› 2006, Vol. 21 ›› Issue (3): 65-70. doi: 10.3321/j.issn:1000-7091.2006.03.016

所属专题： 油料作物；

原生质体非对称融合获得花椰菜与OgucMS甘蓝型油菜种间杂种

惠志明^1,2, 刘凡², 简元才², 申书兴¹, 赵泓²

1. 河北农业大学园艺学院, 河北保定 071001;
2. 国家蔬菜工程技术研究中心, 北京 100089

收稿日期:2005-12-12 出版日期:2006-06-28
通讯作者: 刘凡
作者简介:惠志明（1976- ），男，河北邯郸人，硕士，主要从事芸薹属作物体细胞杂交方面的研究。
基金资助:
农业部948项目（2001-223）；北京市自然科学基金重大项目（5050001）

Interspecific Somatic Hybrids Between Brassica oleracea var botrytis and Ogura TypecMS Brassica napus Obtained from Asymmetric Protoplast Fusion

HUI Zhi-ming^1,2, LIU Fan², JIAN Yuan-cai², SHEN Shu-xing¹, ZHAO Hong²

1. College of Horticultural Sciences, Hebei Agricultural University, Baoding 071001, China;
2. Beijing Vegetable Research Center, Beijing 100089, China

Received:2005-12-12 Published:2006-06-28

摘要/Abstract

摘要： 甘蓝型油菜OgucMS系幼叶原生质体经剂量分别为0.0250，0.0750，0.3150，0.4680和0.9600 J/cm²的紫外线辐照后，与花椰菜下胚轴原生质体通过PEG法诱导融合，其中0.0250~0.4680J/cm²获得再生植株68株，0.9600J/cm²辐射剂量下没有获得再生株。通过根尖染色体计数、流式细胞仪倍性鉴定、同工酶酶谱分析、RAPD及OgucMS线粒体基因orf138 STS（Sequence Tagged Site）标记引物扩增等方法对再生株进行了鉴定。结果表明，其中32株为杂种植株。同时也发现，紫外线辐照剂量的增加对供体染色体的丢失程度影响不大。

关键词: 花椰菜, 非对称融合, OguCMS, 甘蓝型油菜

Abstract: Asymmetric protoplast fusion beween hypocotyl protoplasts of Brassica oleracea var.botrytis and leaf protoplasts of Ogura typecMS Brassica napus was performed by PEG method.Leaf protoplasts from donor plant were treated with UV light at a dose of 0.0250,0.075 0,0.315 0,0.4680 and 0.9600J/cm² respectively.68 plants regenerated in the dose range from 0.0250 to 0.4680J/cm²,and none at the dose of 0.9600J/cm².Results of chromosome counts,ploidy analysis by flow cytometry,isozyme analysis,RAPD and specific amplification(STS primers) of Ogura-CMS-associated mitochondrial gene orF₁38 revealed that 32 somatic hybrid plants were obtained.It was showed in our experiment that the degree of elimination of UV-irradiated donor chromosomes did not depend on the irradiation dose.

Key words: Brassica oleracea var.botrytis, Asymmetric protoplast fusion, OgucMS Brassica napus

中图分类号:

S603.6

惠志明, 刘凡, 简元才, 申书兴, 赵泓. 原生质体非对称融合获得花椰菜与OgucMS甘蓝型油菜种间杂种[J]. 华北农学报, 2006, 21(3): 65-70. doi: 10.3321/j.issn:1000-7091.2006.03.016.

HUI Zhi-ming, LIU Fan, JIAN Yuan-cai, SHEN Shu-xing, ZHAO Hong. Interspecific Somatic Hybrids Between Brassica oleracea var botrytis and Ogura TypecMS Brassica napus Obtained from Asymmetric Protoplast Fusion[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(3): 65-70. doi: 10.3321/j.issn:1000-7091.2006.03.016.

http://www.hbnxb.net/CN/Y2006/V21/I3/65

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原生质体非对称融合获得花椰菜与OgucMS甘蓝型油菜种间杂种

Interspecific Somatic Hybrids Between Brassica oleracea var botrytis and Ogura TypecMS Brassica napus Obtained from Asymmetric Protoplast Fusion

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原生质体非对称融合获得花椰菜与OgucMS甘蓝型油菜种间杂种

Interspecific Somatic Hybrids Between Brassica oleracea var botrytis and Ogura TypecMS Brassica napus Obtained from Asymmetric Protoplast Fusion

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