摘要： 通过测定番茄果实生长发育过程中蔗糖、果糖、可溶性总糖、淀粉的含量和糖代谢中涉及到的蔗糖合成酶、蔗糖磷酸合成酶、酸性转化酶、中性转化酶的活性，研究了番茄果实生长发育过程中糖的代谢过程。根据番茄果实的生长发育曲线和相对日生长率，可以将番茄的生长发育过程按开花后39d作为分界点分为2个阶段，39d之前为产量形成期，之后为品质形成期。在产量形成期内，果糖和可溶性总糖含量逐渐升高，淀粉含量逐渐下降，蔗糖含量先升高后下降，变化不剧烈，以积累淀粉为主。同期AI和NI的活性较低，SS和SPS的活性较高。在品质形成期，果糖含量升高，淀粉含量下降，蔗糖和可溶性总糖变化不明显，以积累果糖为主。AI和NI的活性急剧升高，SS和SPS的活性很低。统计分析表明，番茄果实单果重量与淀粉含量、淀粉含量与NI活性呈显著负相关，可以参考淀粉的含量和NI的活性进行番茄高产研究。果糖含量与淀粉含量呈显著负相关，与SS和AI，NI的活性均为显著正相关；番茄果实中的可溶性总糖与淀粉含量呈显著负相关，与SS的活性呈显著正相关，可以参考SS和AI，NI的活性进行高果糖含量的品质研究。

关键词: 番茄果实, 糖代谢, 蔗糖代谢关键酶

Abstract: The contents of different sugars(sucrose,fructose,total soluble sugar and starch) and the activities of sucrose phosphate synthase,sucrose synthase acid invertase,neutral invertase involved in sucrose metabolism was measured in order to know the sugar metabolism in developing tomato fruit.According to the development and the relative growth rate of tomato fruit,we can devide the tomato fruit development into two PHA_Ses.The first PHA_Se before 39 days after anthysis forms the main output and in the PHA_Se after this the inner quality forms.In the first PHA_Se the contents of fructose and soluble total sugar increase gradually,the content of starch decreases and sucrose's content changes little.At the same time the activities of sucrose synthase and sucrose phosphate synthase relatively high,the invertases'activity relatively low.In the second PHA_Se,the content of fructose increase,the starch's content decrease and the contents of total soluble sugar and sucrose change little.The activities of invertases rise acutely,but the activities of sucrose synthase and sucrose phosphate synthase maintain very low and change little.By statistic analysis,we find the single fruit weight and the content of starch have significant correlation,while the content of starch has significant correlation with neutral invertase,so we can consult the content of starch and the activity of neutral as the index to gain high output.The contents of fructose、total soluble sugar and starch have the significant correlation,the contents of fructose and the activity of SS,AI,NI have significant correlation.The contents of total soluble sugar and the activity of sucrose synthase have significant correlation,so we can consult the activity of synthase and invertases as the index to gain high contents of fructose.

Key words: Tomato fruit, Sugar metabolism, Main enzymes in sucrose metabolism

中图分类号: 

• S641.2