华北农学报 ›› 2006, Vol. 21 ›› Issue (2): 57-61. doi: 10.3321/j.issn:1000-7091.2006.02.014

所属专题: 油料作物

• 论文 • 上一篇    下一篇

甘蓝型黄籽油菜分子标记辅助选择的效果分析

刘志文1,2, 王英1, 刘雪平2, 傅廷栋2, 薛永常1, 涂金星2, 马朝芝2   

  1. 1. 大连轻工业学院生物与食品工程学院, 辽宁大连 116034;
    2. 华中农业大学作物遗传改良国家重点实验室, 湖北武汉 430070
  • 收稿日期:2005-09-28 出版日期:2006-04-28
  • 通讯作者: 傅廷栋
  • 作者简介:刘志文(1972- ),男,湖北罗田人,博士,副教授,主要从事甘蓝型黄籽油菜的育种和分子生物学研究工作。
  • 基金资助:
    国家“973”项目(2001CB108);辽宁省教育厅项目(05L075)

The Effects Analysis of Molecular Marter-assisted Selection in Yellow-seeded Brassica napus

LIU Zhi-wen1,2, WANG Ying1, LIU Xue-ping2, FU Ting-dong XUE Yong-chang2, TUJin-xing1, MA Chao-zhi2   

  1. 1. College of Bio & Food Technology, Dalian Institute of Light Industry, Dalian 116034, China;
    2. National Key Lab of Crop Genetic Improvement, Huazhong Agricultural University, Wuhan 430070, China
  • Received:2005-09-28 Published:2006-04-28

摘要: 应用分子标记辅助选择的方法,将黄籽性状导入到波里马恢复系中,利用杂种优势,将是提高油菜产量的理想途径。分析了分子标记辅助选择甘蓝型黄籽油菜的前景和背景选择的效果,结果表明,在背景选择中,7种不同RAPD引物数目组合方式的选择结果基本一致;应用少数连锁群上的标记可以获得与全基因组相似的选择结果。在前景选择中,随着世代的增加,每一个标记的选择准确率在降低;双侧标记的选择准确率比单侧的标记高,此外,多个单侧标记未能提高选择准确率。据此,提出了作物前景和背景选择过程中的一些改进策略。

关键词: 甘蓝型黄籽油菜, 分子标记, 前景选择, 背景选择

Abstract: To transfer the yellow-seeded gene into Polcms restorer lines and utilize heterosis using molecular marterassisted selection (MAS) would be an ideal approach to increase the rapeseed oil yield.This paper analyzed the effects of the back and foreground selection in yellow-seeded Brassica napus,the results showed that the selection results of 7 different RAPD primer combinations are identical and selection by a limited linkage groups can get the whole genome similar effect in background selection.During foreground selection program,it demonstrated that the selection efficiency of each marker would decrease with generation and the unilateral markers can't increase the efficiency with number,moreover,flanked markers can attain higher efficiency than unilateral markers.As a result,we proposed some improved strategies for back and foreground selection in crop MAS.

Key words: Yellow-seeded Brassaca rzapas, Molecular marker, Background selection, round selection

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引用本文

刘志文, 王英, 刘雪平, 傅廷栋, 薛永常, 涂金星, 马朝芝. 甘蓝型黄籽油菜分子标记辅助选择的效果分析[J]. 华北农学报, 2006, 21(2): 57-61. doi: 10.3321/j.issn:1000-7091.2006.02.014.

LIU Zhi-wen, WANG Ying, LIU Xue-ping, FU Ting-dong XUE Yong-chang, TUJin-xing, MA Chao-zhi. The Effects Analysis of Molecular Marter-assisted Selection in Yellow-seeded Brassica napus[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2006, 21(2): 57-61. doi: 10.3321/j.issn:1000-7091.2006.02.014.

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