摘要： 采用DM-His法对家兔精子进行体外获能处理，利用活体冲卵法从家兔输卵管中冲取卵子，在体外完成获能精子与卵子的受精过程。并将发育的受精卵移植于同期处理的受体兔，而生产试管兔。本试验结果为：350枚家兔卵子体外受精后有179枚发育到2~4细胞，试验组卵裂率达63.15%。将发育到2-4细胞的14枚胚胎移植于受体兔后，获得一只试管兔。
关键词:
精子获能,
体外受精,
试管兔
Abstract: In Present study,DM-His method was applied to external capacitate rabbit sperm which were obtained from oviduct through vital leaching.The capacitated sperm were in vitro fertilised with ovum and transplanted to the simulteniously treated donee to produce tube rabbit,The result showed that 179 ovum among the total 350 inseminated developed into 2-4 cells stage and cleavage rate was 63.15%.14 embryoes which had 2-4 cells,weretransfered into donee,and 1 tube rabbit had been got.
Key words:
Sperm capacitation,
In vitro fertilization,
Tube rabbit
中图分类号:
任克良, 罗惠娣, 毛杨毅, 梁茂文, 韩一超, 梁全忠. 家兔体外受精技术研究[J]. 华北农学报, 2000, 15(1): 135-138. doi: 10.3321/j.issn:1000-7091.2000.01.028.
REN Keliang, LUO Huidi, MAO Yangyi, LIANG Maowen, HAN Yichao, LIANG Quanzhong. A Study on Rabbit External Fertilization[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2000, 15(1): 135-138. doi: 10.3321/j.issn:1000-7091.2000.01.028.