摘要： 研究结果表明,利用吸水纸种植法,白菜黑腐病种子带菌检验只需三步就能完成.1.子叶上“V”字形病斑的出现;2.病斑切开后有菌液渗出;3.在淀粉牛肉汁琼脂培养基上,发亮的淡黄色菌落周围有淀粉水解晕斑.与目前广泛应用的水琼脂种植检验法和洗涤检验法比较,此法经济、简便、有效.

关键词: 白菜黑腐病, 种子带菌检验

Abstract: A new technique was developed to detect Xanthomonas campestris pv. campestris in Brassica seeds. Compared to the commonly used washing test, this technique had shown to be more reliable and economical for routine screening. The technique described here was especially useful under conditions of low infection and in cases where only small amounts of material were available. Although a longer incubation time was required for this technique, the actual labor involved was much lower than that of necessarily required for other methods.

Key words: Seed detection, X anthomonas campestris pv. Campestris