摘要： 研究了以“黄瓜子叶法”为特征的蔬菜灰霉病杀菌剂的筛选模式.采用本方法标准药剂速克灵、扑海因获得很好的重复性|接种最适宜的分生孢子浓度为1×10~5/ml|菌丝菌龄对实验结果有明显的影响|采用浸渍法或喷雾法进行药剂处理对实验结果影响不大,但前者操作似更简单.供试乳化剂、溶剂中,除农乳700号及二甲基甲酰胺之外,对蔬菜灰霉病菌均无明显的影响.
关键词:
蔬菜灰霉病,
生物测定法,
杀菌剂
Abstract: The bioassay model of fungicide characterized by "cucumber cotyledon method" against the grey mold of cucumber caused by Botrytis cinerea has been studied. The repeatability of the experimental result is satisfactory by using this method with the chemicals procymidone and Roval.It has been found that the optimum conidium concentration for inoculation is 1×105 spores/ml, while no disease is produced with the concentration less than 1×102 spores/ml. The age of hypha has significant effect on the result of inoculation, the younger the hypha, the bigger the leaf spot induced.Approximate results have been obtained by using either macerating or spraying method, but the former seems to be even simpler in operation. The surface active agent and solvent used in testing the fungicide has no inhibition effect on B, cinerea except Nong Ru 700 and DMF.
Key words:
Gray mold of cucumber,
Bioassay,
Fungicide
李树正, 张素华, 刘淑芬, 包如棣, 张瑞贤. 黄瓜子叶法筛选蔬菜灰霉病杀菌剂的研究[J]. 华北农学报, 1991, 6(3): 94-99. doi: 10.3321/j.issn:1000-7091.1991.03.017.
LiShuzheng, ZhangSuhua, LiuShufen, BaoRudi, ZhangRuixian. Simple Bioassay Method of New Fungicide Against Botrytis cinerea-Cucumber Cotyledon Method[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1991, 6(3): 94-99. doi: 10.3321/j.issn:1000-7091.1991.03.017.