摘要： 用旋毛虫感染性的幼虫和排泄分泌抗原免疫BALB/C纯系小鼠,取其脾细胞和SP₂/O—Ag14骨髓瘤细胞,在聚乙二醇(PEG)作用下融合,用间接酶联免疫吸附试验(ELISA)检测,阳性孔用有限稀释法经3～4次克隆化筛选培养,获得了D₄G₆和E₂B₁₀二个杂交瘤细胞系.经3～6个月的传代培养、冻存和接种小鼠诱生腹水,杂交瘤细胞生长良好,保持了稳定分泌抗体的能力.鼠性腹水和血清效价分别为6×10^-3和1×10^-4.抗体免疫球蛋白用免疫琼脂双扩散试验测定均为IgG₁亚类.

关键词: 旋毛虫, 单克隆抗体, 杂交瘤细胞系, 细胞融合

Abstract: BALB/C mouse was immunized with infective T.Spiralis larvae. Fusion of spleen cells and SP₂/O—Ag14 were performed in the presence of polyethylene(PEG). Cultures were tested by indirect enzyme-linked immu-nosorbent assay(ELISA). Positive wells were cloned 3-4 times by limited dilution. Two hybrid cell lines-D₄G₆and E₂B₁₀were received and have the ability of secreting antibody steadily and grow very well, which has been proved by the experiment of 3-6 months, such as cell culturing, superfrozen storage and inducing abdominal liquid of inoculated mouse. The titer from mouse abdominal liquid is 6×1^-3 and that from serum of mouse is 10^-4. The antiboby both from D4G6 and E2B10 is IgG1 that was tested by agar bidirectional diffusion.

Key words: Trichinella spiralis, Monoclonal antiboby, Hybrid cell line, Cell fusion