链霉素金标单克隆抗体快速检测试纸的研制及其特性鉴定

doi:10.7668/hbnxb.2010.05.018

华北农学报 ›› 2010, Vol. 25 ›› Issue (5): 85-89. doi: 10.7668/hbnxb.2010.05.018

链霉素金标单克隆抗体快速检测试纸的研制及其特性鉴定

刘庆堂, 职爱民, 邓瑞广, 杨艳艳, 杨继飞, 柴书军, 张改平

河南省农业科学院, 农业部动物免疫学重点开放实验室, 河南省动物免疫学重点实验室, 河南, 郑州, 450002

收稿日期:2010-08-25 出版日期:2010-10-28
作者简介:刘庆堂(1970-),男,河南内黄人,主要从事动物性食品安全生物技术研究.
基金资助:
国家"863"项目资助(2001AA249030)

Development and Traits Identification of Colloidal Gold-Labeled Monoclonal Antibody Rapid Detection Strip of Streptomycin

LIU Qingtang, ZHI Aimin, DENG Ruiguang, YANG Yanyan, YANG Jifei, CHAI Shujun, ZHANG Gaiping

Key Laboratory for Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agriculture Sciences, Zhengzhou 450002, China

Received:2010-08-25 Published:2010-10-28

摘要/Abstract

摘要： 建立了分泌抗链霉素单克隆抗体(STR mAb)杂交瘤细胞株并生产了STR mAb,采用胶体金免疫层析试验(GICA)技术成功研制出STR残留快速检测试纸(STR-Strip),并对其性能进行了鉴定.结果显示,目测检测限为12. μg/kg;与氯霉素等药物无交叉反应;其敏感性与竞争ELISA试剂盒相当,符合率为100%.研制的快速检测试纸可以在10 min内显示结果,具有快速、敏感、特异、简便等特点,适合STR残留的现场快速检测.

关键词: 链霉素, 金标快速检测试纸, 单克隆抗体, 特性鉴定

Abstract: For the development of colloidal gold-labeled monoclonal antibody rapid detection strip of streptomycin,the monoclonal antibody against streptomycin was generated and the rapid test strip of streptomycin(STR-Strip) was developed by gold immunochromatography assay(GICA) . Its traits were tested. The results showed that the detection limit of STR-Strip was 12. 5 μg /kg by eyes,it had no cross-reactivity towards other antibacterials,its sensitivity was as same as that of competitive ELISA-Kit and its coincidence rate was 100% compared with ELISA-Kit. With the strip,the test results were showed in less than 10 min,the strip possessed sensitivity,specificity and briefness. The strip was proved to be used for the rapid test of STR residues in animal food.

Key words: Streptomycin, Gold-labeled rapid detection strip, Monoclonal antibody, Traits identification

中图分类号:

刘庆堂, 职爱民, 邓瑞广, 杨艳艳, 杨继飞, 柴书军, 张改平. 链霉素金标单克隆抗体快速检测试纸的研制及其特性鉴定[J]. 华北农学报, 2010, 25(5): 85-89. doi: 10.7668/hbnxb.2010.05.018.

LIU Qingtang, ZHI Aimin, DENG Ruiguang, YANG Yanyan, YANG Jifei, CHAI Shujun, ZHANG Gaiping. Development and Traits Identification of Colloidal Gold-Labeled Monoclonal Antibody Rapid Detection Strip of Streptomycin[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2010, 25(5): 85-89. doi: 10.7668/hbnxb.2010.05.018.

http://www.hbnxb.net/CN/Y2010/V25/I5/85

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链霉素金标单克隆抗体快速检测试纸的研制及其特性鉴定

Development and Traits Identification of Colloidal Gold-Labeled Monoclonal Antibody Rapid Detection Strip of Streptomycin

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