摘要： 用二倍体多年生类玉米、玉米自交系330及杂种F1（玉米自交系330×二倍体多年生类玉米）的幼雄穗进行离体培养，获得高频率的植株再生。幼雄穗培养在含2mg/16-BA的MS培养基上直接产生不定芽。在这一培养基上继代培养，建立了不定芽的快速无性繁殖系。不定芽转移到生根培养基（MS附加0.5mg/lNNA，300mg/l水解乳蛋白和1000mg/l活性碳），非常容易生根。幼雄穗的发育阶段和植物激素的配比，对培养物的成功建立是非常重要的。本文还讨论了这一技术的应用。
关键词:
二倍体多年生类玉米,
幼雄穗离体培养,
不定芽
Abstract: Plants were regenerated with high frequency from immature tassels of Zea mays L.(maize inbred 330),Zea diplopetennis,and Zea maysZea×Zea diplopennis hybrid cutured in vitro.Adventitious buds and shoots were directly developed in the primary culture from the immature tassels cultured on the medium MS containing 2 mg/1 6-BA.Subcultured on the same medium,rapid,clonal bud and shoot propagation was obtained.The buds and shoots were very easy of rooting by transferring them to root-inducing medium,MS containing 0.5 mg/1 NAA,300 mg/1 lactalbumin hydrolyzate(LH),and 1000 mg/1 activated charcoal.The developmental stage of immature tassels and the combination of phytohormones were most important for successful culture establishment.The application of this technique was discussed.
Key words:
Zea diploperennis,
Immature tassel culture in vitro,
Direa formation of adventitious buds and shoots
郭骁才, 张玉玲. 二倍体多年生类玉米及其种间杂种幼雄穗的离体培养 Ⅰ.不定芽的直接形成和植株再生[J]. 华北农学报, 1988, 3(4): 16-22. doi: 10.3321/j.issn:1000-7091.1988.04.004.
Guo Xiaocai, Zhang Yuling. In Vitro Culture of Immature Tassels of Zea diploperemis and Its Interspecific Hybrid.Ⅰ.Direct Formation of Adventitious Buds and Plant Regeneration[J]. ACTA AGRICULTURAE BOREALI-SINICA, 1988, 3(4): 16-22. doi: 10.3321/j.issn:1000-7091.1988.04.004.