摘要： 本研究以旋毛虫可溶性抗原作诊断抗原，聚苯乙烯微球为抗原载体，通过碳化二亚胺反应把旋毛虫抗原共价偶联到聚苯乙烯载体微球上，制备了用于检测猪旋毛虫病血清抗体的免疫微球诊断试剂。从平板凝聚试验技术对117份实验感染旋毛虫病的猪、兔、大白鼠的血清抗体和疫区猪血清抗体进行了检测。试验结果，免疫微球凝集试验对实验感染旋毛虫病动物血清抗体检测的敏感性和特异性均为100%。在检测的敏感性上，免疫微球凝集试验比人工消化检查法高9.17%，比压片镜检法高25.26%。在阳性符合率上，与压片镜检相比为100%，与人工消化检查相比为97.25%。试验结果表明，免疫微球凝集试验技术对猪旋毛虫病是一简便、快速和可靠的诊断方法。

关键词: 旋毛虫, 猪旋毛虫病, 免疫微球, 可溶性抗原, 碳化二亚胺反应, 共价偶联

Abstract: Immuno-microspheres for detection of swine trichinosis had been prepared from soluble antigen of trichinella spiralis covalently bound to polystyrene micro-spheres by carbodiimide reaction.117 sera of pigs,rabbits and rats infected experimentally with trichinella spirallis from our Laboratory and sera of pigs from epidemic area were detected with the immuno-microsphere agglutination test.Detecting results showed that the immuno-microsphere agglutination test had a sensitivity of 100% and a specificity of 100% for detection of animal trichinosis infected experimentally.The positive rates of identity of the immuno-microsphere agglutination test were 100% and 97.25% identical with that microscopic section and digestion of muscles for postslaughter respectively.On the positive rates of detection the sensitivity of immuno-microsphere agglutination test increased 9.17% and 25.26% compared with that of digestion and microscopic section of muscles for postslaughter respectively.It has been demonstrated that the immuno-microsphere agglutination test is a simple,quick,sensitive and specific serological method for detection of swine trichinosis.

Key words: Trichinella spirallis, Swine trichinosis, Immuno-microspheres, Soluble antigen, Carbodiimide reaction, Covalent bound