Cloning and Expression Pattern of DNA Methyl-binding Domain Gene PsMBD5 in Tree Peony
SI Fuhui1, ZHANG Yuxi1, LIU Chunying1, GAI Weiling2, ZHAN Xinmei1, GAI Shupeng1
1. College of Life Sciences, Qingdao Agricultural University, Key Lab of Plant Biotechnology in Universities of Shandong Province, Qingdao 266109, China;
2. College of Agronomy and Plant Protection, Qingdao Agricultural University, Qingdao 266109, China
Abstract：The previous results of the transcriptome and expression profiling analysis in tree peony found that a MBD-like fragment was differentially expressed during low temperature induced dormancy release.To investigate the characteristic and putative function of MBD during tree peony bud dormancy release,total 1 204 bp full length cDNA of PsMBD5 was obtained by RACE amplification.The coding fame of 1 056 bp encoded 351 amino acids.The result of bioinformatics analysis showed that the molecular weight of PsMBD5 was 38.127 4 kDa,isoelectric point was 5.14,which indicated that PsMBD5 was an unstable hydrophilic.The result of homology analysis indicated that the similarity between the PsMBD5 and PmMBD5 was the highest with 38.1% identity,and CsMBD5 was the lowest with 25.8%.When compared with 13 kinds of AtMBD,the identity between the PsMBD5 and AtMBD5 was the highest.The expression patterns were analyzed by Real-time quantitative PCR,and the results showed that the transcript level of PsMBD5 at the early stage of flowering in root was the highest,followed by in petals,carpel and sepals,and in stamen was the lowest.During the whole process of chilling fulfillment, PsMBD5 was highly induced by low temperature.The transcript level of PsMBD5 was the highest after 14 d chilling fulfillment,and maintained a high level of transcription after that.These results would provide theoretical basis to validate the regulation mechanism of PsMBD5 during the peony bud dormancy release.
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2017, Vol. 32 
