Cloning and Bioinformatics Analysis on CDS of FAM134B Gene in Yak
ZENG Guo-min1,3, DU Xiao-hua2,3, YANG Yang2, LIANG Chun-hua2, SUN Xue-jing2, LIU Xia1,3
1. College of Life Science and Technology, Gansu Agricultural University, Lanzhou 730070, China;
2. College of Veterinary Medicine, Gansu Agricultural University, Lanzhou 730070, China;
3. Gansu Key Laboratory of Herbivorous Animal Biotechnology, Lanzhou 730070, China
Abstract：In order to enrich basic data in yak FAM134B gene and further explore the physiological function of FAM134B gene.By use of the PCR amplification and sequencing and bioinformatics analysis software to complete the yak FAM134B gene cloning,sequencing and bioinformatics analysis.The 1 079 bp length FAM134B gene in yak was got by cloning,including the 1 071 bp length CDS region(GenBank accession No.:KM587693),and encoding 356 amino acids.Alignment with CDS and amino acid sequence of cattle FAM134B gene,three base mutations were found.The 727th base C was mutated to T and caused codon CCC variable TCC encoding amino acid from proline to serine.The formula of protein encoded by FAM134B gene in yak was C1705H2686N448O575S13,and the molecular weight was 39.077 5 kDa,the theory isoelectric point was 4.46,the extinction coefficient was 24 450,the instability index was 46.85,the aliphatic index was 79.47,and the grand average of hydropathicity was-0.439.It was an unstable and soluble protein.The secondary structure of FAM134B was mainly α-helices and random coil,α-helices was 23.88% and random coil was 74.72%,FAM134B was belongs to mixed type of protein.Subcellular localization of FAM134B was in the endoplasmic reticulum(30.4%), plasma membrane(21.7%),vacuolar(17.4%),nuclear(17.4%),golgi(4.3%),cytoskeletal(4.3%),and mitochondrial(4.3%),speculated that it might play ion channel carrier and signal transduction and transcriptional regulation in material transport and cofactor biosynthesis process.The amino acid homology of FAM134B compared Yak to Bos taurus,Ovis aries,Mus musculus,Rattus norvegicus,Macaca mulatta,Pan troglodytes,Homo and Xenopus laevis sapiens were 99.7%,97.2%,87.1%,86.8%,90.4%,90.2%,90.4%,57.9%.There was a high homology among different species,and phyletic evolution were same as their genetic relationship.The research indicated that the FAM134B gene coding region was conservative in the course of evolution.The gene was cloned and analyzed,which provided the theoretical basis for revealing the genetic characteristics of yak FAM134B gene.
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2015, Vol. 30 
