Cloning and Bioinformatics Analysis of TSP1 and TSP6 Gene of Echinococcus granulosus
LIU Tian-li1, MENG Qing-ling1, QIAO Jun1, CHEN Cheng1, MA Yu1, HU Zheng-xiang1, CAI Xue-peng2, CHEN Chuang-fu1
1. College of Animal Science and Technology, Shihezi University, Shihezi 832003, China;
2. Lanzhou Veterinary Research Institute, Chinese Academy of Agricultural Sciences, Lanzhou 730046, China
Abstract：In order to study the function of two important antigen genes tetraspanin 1-TSP1(TSP1) and tetraspanin 1-TSP6(TSP6), primers derived from Echinococcus granulosus genome database in GenBank were designed and the open reading frame(ORF) sequences of TSP1 and TSP6 were cloned by RT-PCR from hydatid protoscolex.Then they were cloned into pMD19-T vector for bioinformatics analysis.The results indicated that the TSP1 cDNA contains 792 nucleotides.The deduced protein consisted of 263 amino acids and has three N-glycosylation sites, two N-acylation sites.The gene sequence showed about 98.99% identity with the TSP1 (EG_11043) reported and the induced amino acid sequence showed about 98.48% identity.The TSP6 cDNA contains 666 nucleotides.The deduced protein consisted of 221 amino acids and has five N-acylation sites, one Tyrosine kinase phosphorylation sites.The gene sequence showed about 98.18% identity with the TSP6 (EG_00715) reported and the induced amino acid sequence showed about 85.07% identity.The study carried out bioinformatics analysis of the TSP1 and TSP6 gene of Eg by molecular biology software to predict the structure and epitope of protein antigens known and laid a good foundation for the preparation of developing a vaccine.
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2015, Vol. 30 
