Abstract：In this study,we used the strains F1-40(Mating type A)and 01-23(Mating type a)as materials.In order to obtain the full-length and the flanking sequence of MAT genes,the MAT1 gene of mating type A strain and the MAT2 gene of mating type a strain of Setosphaeria turcica were cloned by PCR and genome walking with the candidate gene strategy.The conserved domain database of the full-length genes were analyzed.The flanking sequence of MAT1 and MAT2 genes were comprised by the Blast of genomic database of S.turcica. The results suggested that both genes contain one introns.MAT1 encodes amino acids contain a complete MAT α-domain,the domain as part of the MAT alpha1 family.MAT2 gene encodes amino acids,including a complete MATA HMG-box combining with domain,the domain as part of the DNA binding protein in the HMG-box Ⅰ class members of the family,such member containing a single HMG box,composed of three helix,which located between the 133-202 residues in the form of highly sequence specificity combined with DNA minor groove participating in the process of copy transcription and translation of DNA.Then participate in the process of sexual reproduction.Comparing the flanking sequence of MAT1 and MAT2 genes,we discovered the similarity was higher than 93%.
[1] Debuchy R,Turgeon B G.The mycota I.Growth,differentiation,and sexuality[M].Germany,Berlin:Springer-Verlag,2006:293-323.
[2] Hicks J,Strathern J N,Klar A J.Transposable mating type genes in Saccharomyces cerevisiae [J].Nature,1979,282(5378):473-478.
[3] Guzman D,Garber R C,Yoder O C.Cytology of meiosis I and chromosome number of Cochliobolus heterostrophus(Ascomycetes)[J].Canadian Journal of Botany,1982,60:1138-1141.
[4] Mandel M A,Barker B M,Kroken S,et al.Genomic and population analyses of the mating type loci in Coccidioides species reveal evidence for sexual reproduction and gene acquisition[J].Eukaryotic Cell,2007,6(7):1189-1199.
[5] Turgeon B G,Bohlmann H,Ciuffetti L M,et al.Cloning and analysis of the mating type genes from Cochliobolus heterostrophus [J].Molecular and General Genetics,1993,238(1/2):270-284.
[6] Barve M P,Arie T,Salimath S S,et al.Cloning and characterization of the mating type(MAT) locus from Ascochyta rabiei(Teleomorph: Didymella rabiei) and a MAT phylogeny of legume-associated Ascochyta spp.[J].Fungal Genetic Biology,2003,39(2):151-167.
[7] McGuire I C,Marra R E,Turgeon B G,et al.Analysis of mating-type genes in the chestnut blight fungus, Cryphonectria parasitica [J].Fungal Genetics and Biology,2001,34(2):131-144.
[8] Nolting N,Pggeler S.A MADS box protein interacts with a mating-type protein and is required for fruiting body development in the homothallic ascomycete Sordaria macrospora [J].Eukaryotic Cell,2006,5(7):1043-1056.
[9] Turgeon B G,Sharon A,Wirsel S,et al.Structure and function of mating type genes in Cochliobolus spp.and asexual fungi[J].Canadian Journal of Botany,1995,73(S1):783-788.
[10] Sharon A,Yamaguchi K,Christiansen S,et al.An asexual fungus has the potential for sexual development[J].Molecular and General Genetics,1996,251(1):60-68.
[11] Yamada-Inagawa T,Klar A J,Dalgaard J Z. Schizosac-charomyces pombe switches mating type by the synthesis-dependent strand-annealing mechanism[J].Genetics,2007,177(1):255-265.
[12] Houston P L,Broach J R.The dynamics of homologous pairing during mating type interconversion in budding yeast[J].Public Library of Science Genetics,2006,2(6):e98.
[13] Valencia-Burton M,Oki M,Johnson J,et al.Different mating-type-regulated genes affect the DNA repair defects of Saccharomyce s RAD51,RAD52 and RAD55 mutants[J].Genetics,2006,174(1):41-55.
[14] Menkis A,Jacobson D J,Gustafsson T,et al.The mating-type chromosome in the filamentous ascomycete Neurospora tetrasperma represents a model for early evolution of sex chromosomes[J].Public Library of Science Genetics,2008,4(3):e1000030.
[15] Yun S H,Berbee M L,YODER O C,et al.Evolution of the fungal self-fertile reproductive life style from self-sterile ancestors[J].Proceedings of the National Academy of Sciences of the United States of America,1999,96(10):5592-5597.
[16] Patrik I,Jennifer H B,Gillian T,et al.Lateral transfer of mating system in Stemphylium[J].Proceedings of the National Academy of Sciences of the United States of America,2005,102(32):11390-11395.
[17] Wik L,Karlsson M,Johannesson H.The evolutionary trajectory of the mating-type(mat) genes in Neurospora relates to reproductive behavior of taxa[J].BMC Evolutionary Biology,2008,8:109.
2014, Vol. 29 
