连翘UGT基因家族全基因组鉴定及其在不同产地的表达分析

doi:10.7668/hbnxb.20196417

华北农学报 ›› 2026, Vol. 41 ›› Issue (4): 75-86. doi: 10.7668/hbnxb.20196417

所属专题： 生物技术；

• 作物遗传育种·种质资源·生物技术 • 上一篇下一篇

连翘UGT基因家族全基因组鉴定及其在不同产地的表达分析

王宁¹, 陈雪¹, 姚朝杰¹, 杜晨晖¹, 杨琬芳², 李永³, 王宏伟¹^,⁴, 张瑜¹

¹ 山西中医药大学中药与食品工程学院, 山西晋中 030619
² 山西中医药大学基础医学院, 山西晋中 030619
³ 内蒙古师范大学生命科学与技术学院, 内蒙古呼和浩特 010022
⁴ 山西医科大学第二临床医学院, 山西太原 030001

收稿日期:2025-09-08 出版日期:2026-08-28
通讯作者:
张瑜(1982—),女,山西晋中人,副教授,博士,主要从事分子生药学研究。
王宏伟(1966—),男,山西忻州人,教授,博士,主要从事血液病中西医结合基础研究。
作者简介:
王宁(1999—),男,内蒙古呼和浩特人,在读硕士,主要从事中药药理学与分子生药学研究。
基金资助:
山西中医药大学2025年研究生科研实践创新项目(X2025KY023); 山西省教育厅2025年度研究生教育创新计划研究生学术创新项目(2025XS486); 山西省科学技术厅山西省科技创新人才团队项目(202304051001020); 山西省中医药重点研究室建设项目(zyyyjs2024024); 山西省中医药管理局中医药创新团队项目(zyytd2024026); 山西中医药大学科技创新团队项目(2022TD2009); 山西中医药大学科技创新能力培育计划(2024PY-JL-10-01); 山西中医药大学博士科研启动资金(2023BK41); 2025年度中医药产业发展现代化支撑项目(2025zyycy02); 血液病中西医结合基础研究(2025XK30)

Genome-wide Identification of the UGT Gene Family in Forsythia suspensa and Its Expression Analysis in Different Regions

WANG Ning¹, CHEN Xue¹, YAO Chaojie¹, DU Chenhui¹, YANG Wanfang², LI Yong³, WANG Hongwei¹^,⁴, ZHANG Yu¹

¹ College Pharmaceutical and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong 030619, China
² College of Basic Medical Sciences, Shanxi University of Chinese Medicine, Jinzhong 030619, China
³ College of Life Science and Technology, Inner Mongolia Normal University, Hohhot 010022, China
⁴ The Second Clinical Medical School of Shanxi Medical University, Shanxi Medical University, Taiyuan 030001, China

Received:2025-09-08 Published:2026-08-28

语音导读.mp3(696KB)

摘要/Abstract

摘要：

为探索山西连翘道地性形成的分子机制,基于连翘基因组数据,采用生物信息学方法对连翘UGT基因家族成员进行了全基因组鉴定和特征分析;并利用不同产地连翘转录组数据和实时荧光定量PCR技术分析了连翘UGTs基因在不同产地和不同组织中表达模式。结果表明,在连翘全基因组中共鉴定到170个家族成员,该家族成员不均匀地分布在14条染色体上,分别命名为FsUGT1~FsUGT170;其编码的蛋白分子质量为11.16~56.77 ku,等电点为4.70~9.18,其中,大多数为亲水性蛋白。亚细胞定位预测结果显示,FsUGTs蛋白主要定位于叶绿体和细胞质中。系统进化树分析发现,连翘UGT基因家族可划分为15个亚组;共线性分析发现,23个FsUGTs和拟南芥的20个UGTs组合形成31种共线性关系。基因结构、蛋白质基序和保守结构域分析显示,FsUGTs基因具有1~7个外显子和0~6个内含子,编码的蛋白均具有PSPG保守结构域。启动子顺式作用元件分析发现,FsUGTs基因启动子区域含有多个光响应、植物激素响应、生物和非生物胁迫以及生长发育相关等元件。基因表达模式分析发现,FsUGT4和FsUGT145在山西连翘中的表达量显著高于河南和陕西,并在连翘叶中的表达量显著高于花和果实。综上,研究结果鉴定了170个连翘UGT基因家族成员,并筛选得到2个在山西连翘高表达并推测与连翘苷合成相关的基因FsUGT4和FsUGT145。

关键词: 连翘, UGT基因家族, 全基因组鉴定, 生物信息学分析, 表达分析

Abstract:

To explore the formation molecular mechanism of the geoherbalism of Shanxi Forsythia suspensa,a genome-wide identification and characterization of UGT gene family members was performed using bioinformatics methods based on the genome data of F.suspensa.Transcriptome data of F.suspensa from different producing areas and Real-time PCR were used to analyze the expression patterns of UGTs gene in F.suspensa from different habitats and different tissues.The results showed that a total of 170 family members were identified in the whole genome of F.suspensa,and the family members were unevenly distributed on 14 chromosomes,named as FsUGT1—FsUGT170.The molecular weight of 11.16—56.77 ku and isoelectric point of 4.70—9.18,most of which are hydrophilic proteins.Subcellular localization predictions indicated that FsUGT proteins were predominantly localized in the chloroplast and cytoplasm.Phylogenetic analysis classified the F.suspensa UGT gene family into 15 distinct subgroups.Synteny analysis identified 31 collinear gene pairs involving 23 FsUGTs and 20 UGTs from Arabidopsis thaliana.Analysis of gene structure,conserved protein motifs,and conserved domains revealed that the FsUGT genes possess 1 to 7 exons and 0 to 6 introns,and all encoded proteins contain the characteristic PSPG conserved domain.Promoter cis-acting element analysis demonstrated the presence of multiple regulatory elements associated with light responsiveness,phytohormone signaling,biotic and abiotic stress responses,and growth and development within the promoter regions of FsUGT genes.Gene expression profiling showed that the transcript levels of FsUGT4 and FsUGT145 were significantly higher in F.suspensa samples from Shanxi compared to those from Henan and Shaanxi,and their expression in leaves was significantly higher than in flowers and fruits.In conclusion,this study identified 170 members of the UGT gene family in F.suspensa and highlighted two genes,FsUGT4 and FsUGT145,which exhibit elevated expression in Shanxi accessions and are potentially implicated in forsythin biosynthesis.

Key words: Forsythia suspensa, UGT gene family, Genome wide identification, Bioinformatics analysis, Expression analysis

中图分类号:

R282.71

王宁, 陈雪, 姚朝杰, 杜晨晖, 杨琬芳, 李永, 王宏伟, 张瑜. 连翘UGT基因家族全基因组鉴定及其在不同产地的表达分析[J]. 华北农学报, 2026, 41(4): 75-86. doi: 10.7668/hbnxb.20196417.

WANG Ning, CHEN Xue, YAO Chaojie, DU Chenhui, YANG Wanfang, LI Yong, WANG Hongwei, ZHANG Yu. Genome-wide Identification of the UGT Gene Family in Forsythia suspensa and Its Expression Analysis in Different Regions[J]. Acta Agriculturae Boreali-Sinica, 2026, 41(4): 75-86. doi: 10.7668/hbnxb.20196417.

http://www.hbnxb.net/CN/Y2026/V41/I4/75

图/表 16

表1 qRT-PCR引物

Tab.1 Sequences of primers used in qRT-PCR

基因名称 Gene name	正向引物(5'—3') Forward primer (5'—3')	反向引物(5'—3') Reverse primer (5'—3')
FsUGT4	CTCCGATGCTGAATTGCACG	ATGGCGCAAGAATTTCGTCG
FsUGT145	TCATGTCGCCTTCCTCCCTA	CCAGGGACATCCGACTTACG
UKN1	CAGACCAGCTTTGAGGAGTATC	GGCCAGAAACCAGTAGTCAATA

图1 连翘UGT基因家族在染色体上的定位

Fig.1 Chromosome location of UGT gene family in F.suspensa

图2 UGT基因家族成员的保守基序和基因结构

Fig.2 The conserved motif and gene structures of the UGT gene family

表2 连翘UGT家族蛋白的理化性质

Tab.2 Physical and chemical properties of UGT family in Forsythia suspensa

项目 Item	氨基酸数目/个 Amino acid number	分子质量/ku Molecular weight	等电点 pI	不稳定系数 Instability index	平均亲水性 Average hydrophilicity	脂溶指数 Aliphatic index
分布范围 Range of distribution	100~509	11.16~56.77	4.70~9.18	22.81~67.82	-0.517~0.458	65.55~106.00
平均值 Mean value	420	47.07	5.89	42.25	-0.143	89.98

图3 连翘UGT基因家族亚细胞定位预测

Fig.3 Prediction of subcellular localization distribution of UGT gene family from Forsythia suspensa

表3 连翘UGT蛋白的二级结构比例

Tab.3 Secondary structure ratio of Forsythia suspensa UGT protein %

项目 Item	α-螺旋 α-helix	延伸链 Extended strand	β-折叠 β-sheet	无规则卷曲 Random coil
分布范围 Range of distribution	19.00~51.22	10.88~26.00	3.45~13.00	26.02~43.77
平均值 Mean value	41.58	14.68	5.56	38.19

图4 连翘UGT蛋白的三级结构预测

Fig.4 Tertiary structure of Forsythia suspensa UGT protein

图5 拟南芥和连翘UGT基因家族成员的系统进化分析

Fig.5 Phylogenetic analysis of the UGT gene family between A.thaliana and F.suspensa

图6 拟南芥与连翘UGT基因的共线性分析

Fig.6 Collinearity analysis of UGT gene in A.thaliana and F.suspensa

图7 连翘UGT基因家族共线性

Fig.7 The collinearity of the UGT gene family in F.suspensa

表4 连翘UGT进化选择压力分析

Tab.4 Evolutionary selection pressure analysis of F.suspensa UGT

基因1 Gene 1	基因2 Gene 2	非同义替换率 Ka	同义替换率 Ks	进化选择压力 Ka/Ks
FsUGT1	FsUGT131	0.121 2	0.724 4	0.167 3
FsUGT8	FsUGT163	0.183 9	1.541 4	0.119 3
FsUGT9	FsUGT170	0.326 6	3.118 1	0.104 7
FsUGT10	FsUGT75	0.204 0	1.455 0	0.140 2
FsUGT53	FsUGT117	0.157 8	1.264 4	0.124 8
FsUGT55	FsUGT126	0.129 9	2.265 8	0.057 3
FsUGT57	FsUGT124	0.149 6	0.732 7	0.204 2
FsUGT61	FsUGT148	0.270 3	1.269 5	0.212 9
FsUGT143	FsUGT157	0.087 2	1.348 9	0.064 6

图8 连翘UGT基因的启动子顺式作用元件分析

Fig.8 Analysis of promoter cis-acting elements of FsUGT genes in F.suspensa

图9 不同产地的连翘UGT基因家族的表达分析

Fig.9 Transcript analysis of UGT gene family in different origins of F.suspensa

图10 FsUGTs与已知功能氧糖基转移酶基因的系统进化分析

Fig.10 Phylogenetic analysis of FsUGTs and known functional O-glycosyltransferase genes

图11 不同产地连翘中FsUGT4和FsUGT145的表达量分析不同小写字母表示不同处理间差异显著(P<0.05)。图12同。

Fig.11 Expression analysis of FsUGT4 and FsUGT145 in F.suspensa from different geographical origins Different lowercase letters indicated significant differences among different treatments(P<0.05).The same as Fig.12.

图12 FsUGT4和FsUGT145基因在不同组织中的表达分析

Fig.12 Expression levels of FsUGT4 and FsUGT145 genes from Forsythia suspensa in different tissues

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连翘UGT基因家族全基因组鉴定及其在不同产地的表达分析

Genome-wide Identification of the UGT Gene Family in Forsythia suspensa and Its Expression Analysis in Different Regions

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连翘UGT基因家族全基因组鉴定及其在不同产地的表达分析

Genome-wide Identification of the UGT Gene Family in Forsythia suspensa and Its Expression Analysis in Different Regions

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