摘要： 小麦是人类的主要粮食作物之一。硫化氢（H₂S）被认为是细胞内第3种气体信号分子，关于H₂S对植物的调控机理的研究越来越多，主要集中在参与植物形态建成，调节生理生化活动，应答非生物胁迫，应答生物胁迫4个方面，但外源H₂S对小麦根系生理生化及蛋白质表达调控作用机制的研究鲜有少见，研究其具有重要意义。采用硫氢化钠作为硫化氢的外源性供体，研究不同浓度硫氢化钠（0，0.05，0.10，0.20，0.40，0.80，1.60，3.20 mmol/L）对小麦根蛋白质差异表达谱的影响，以探寻H₂S对小麦幼苗生长发育的机制。通过对小麦根部的比较蛋白质组学分析发现，不同浓度的硫氢化钠处理引起了涉及光合作用、糖代谢和能量代谢、氧化还原平衡、蛋白质合成、加工与降解，以及信号转导等途径的蛋白质的差异表达。初步推测，硫化氢可能通过调节参与上述代谢途径的功能蛋白质的表达量，进而相应地影响到植株生理与生物量等指标，从而引起植株表型的变化。

关键词: 硫化氢, 小麦根系, 比较蛋白质组, 主成分分析法

Abstract: Wheat is one of the main food crops in human.Hydrogen sulfide(H₂S) is considered to be the cells of the third gaseous signal molecule,more and more research on the regulation mechanism of H₂S on plants,mainly concentrated in the involved in plant morphogenesis,regulation of physiological and biochemical activities,abiotic stress responses four aspects of biological stress response,but few studies of exogenous H₂S on wheat root physiology and protein the expression mechanism of regulation of rare,it is very important to study. In this study,NaHS,an exogenous H₂S donor,was used to determinate changes of the protein expression map,morphological and physiological data in the wheat seedlings under different concentrations of 0,0.05,0.10,0.20,0.40,0.80,1.60,3.20 mmol/L by taking the culture method of nutrient solution.With analysis of the identification of differentially expressed protein in wheat roots. The main impact of different concentrations of H₂S is regulating different kinds of protein expression,such as energy metabolism(glucose metabolism),photosynthesis,enzymatic antioxidant system,protein expression and processing,and signal transduction.Finally it creates the different phenotype of the wheat. We think it is by regulating the expression of different proteins that different concentrations of H₂S promote the growth of wheat at low concentr ations,inhibit at increased concentration and damage when the concentration is too high.

Key words: Hydrogen sulfide, Wheat roots, Comparative proteomics, Principal components

中图分类号: 

• Q78

• S512.03