毛尖紫萼藓类泛素蛋白基因的克隆与表达分析

doi:10.7668/hbnxb.2014.06.011

华北农学报 ›› 2014, Vol. 29 ›› Issue (6): 58-62. doi: 10.7668/hbnxb.2014.06.011

所属专题： 抗旱节水；生物技术；

毛尖紫萼藓类泛素蛋白基因的克隆与表达分析

张梅娟¹, 沙伟^1,2, 刘博², 安洪雪², 宋璐²

1. 东北林业大学生命科学学院, 黑龙江哈尔滨 150040;
2. 齐齐哈尔大学生命科学与农林学院, 黑龙江齐齐哈尔 161006

收稿日期:2014-09-09 出版日期:2014-12-28
通讯作者: 沙伟(1963-),女,黑龙江齐齐哈尔人,教授,博士,博士生导师,主要从事苔藓植物遗传学和分子生物学研究。
作者简介:张梅娟(1982-),女,山东海阳人,在读博士,主要从事苔藓植物分子生物学方面的研究。
基金资助:
国家自然科学基金项目(31070180;31270254);黑龙江省自然科学基金重点项目(ZD201408)

Cloning and Expression Analysis of Ubiquitin-like Protien Gene in Grimmia pilifera

ZHANG Mei-juan¹, SHA Wei^1,2, LIU Bo², AN Hong-xue², SONG Lu²

1. College of Life Science, Northeast Forestry University, Harbin 150040, China;
2. College of Life Science and Agriculture and Forestry, Qiqihar University, Qiqihar 161006, China

Received:2014-09-09 Published:2014-12-28

摘要/Abstract

摘要： 为研究类泛素蛋白基因在苔藓植物中的生物学功能,从毛尖紫萼藓干旱cDNA文库中获得了类泛素蛋白基因cDNA全长序列,命名为 Gp-UBL5。该基因cDNA全长471 bp,包含1个222 bp的完整开放阅读框,编码73个氨基酸组成的蛋白。生物信息学分析表明, Gp-UBL5 基因编码蛋白分子质量为8.525 kDa,等电点为7.84,为稳定型蛋白。进化分析显示,该编码蛋白与二穗短柄草、大麦等类泛素蛋白具有较高一致性,达96%~99%。实时荧光定量PCR结果表明, Gp-UBL5 基因在复水和快速干旱的各个时期均有表达,推测该基因可能在毛尖紫萼藓的抗旱机制中发挥作用。

关键词: 毛尖紫萼藓, 类泛素蛋白基因(Gp-UBL5), 基因克隆, 实时荧光定量PCR

Abstract: In order to study biological function of ubiquitin-like protein(UBL) in bryophytes,A full-length cDNA sequence of ubiquitin-like protein (UBL) gene was obtained from Grimmia pilifera cDNA library,named Gp-UBL5.The full length of Gp-UBL5 was 471 bp,contained a 222 bp open reading frame(ORF),and encoding a protein with 73 amino acids.Bioinformatics analysis suggested that this protein was a stable protein with a calculated molecular mass of 8.525 kDa and a theoretical pI of 7.84.Phylogenetic analysis indicated that Gp-UBL5 protein had 96% to 99% identities with other UBL5 proteins from different species,such as Brachypodium distachyon and Hordeum vulgare.The qRT-PCR result showed that Gp-UBL5 gene could express in rehydration and dehydration.It was speculated that the gene might play an important role in response to drought.These results provided important information for the further study of functional verification.

Key words: Grimmia pilifera, Ubiquitin-like protien gene (Gp-UBL5), Gene clone, Real-time qPCR

中图分类号:

张梅娟, 沙伟, 刘博, 安洪雪, 宋璐. 毛尖紫萼藓类泛素蛋白基因的克隆与表达分析[J]. 华北农学报, 2014, 29(6): 58-62. doi: 10.7668/hbnxb.2014.06.011.

ZHANG Mei-juan, SHA Wei, LIU Bo, AN Hong-xue, SONG Lu. Cloning and Expression Analysis of Ubiquitin-like Protien Gene in Grimmia pilifera[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2014, 29(6): 58-62. doi: 10.7668/hbnxb.2014.06.011.

http://www.hbnxb.net/CN/Y2014/V29/I6/58

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