摘要： 为了减轻氟磺胺草醚对土壤的污染问题,利用富集培养技术从山东某农药厂活性污泥中筛选到1株具有高效降解氟磺胺草醚能力的细菌,命名为BX3。通过菌落形态、生理生化特征和16S rDNA基因序列分析,初步鉴定菌株BX3为微嗜酸寡氧单胞菌。同时对菌株BX3的生长特性及降解特性进行了初步研究。结果表明,该菌株降解氟磺胺草醚的最适条件为:氟磺胺草醚质量浓度为100 mg/L,培养温度为30℃,pH值6～7。在最适条件下,菌株BX3具有较强的降解氟磺胺草醚的能力,培养5 d后对氟磺胺草醚的降解率达到80%以上。可为氟磺胺草醚污染土壤的生物修复提供适宜的菌种资源。
关键词:
氟磺胺草醚,
降解菌,
微嗜酸寡氧单胞菌,
降解特性
Abstract: In order to decrease the amount of fomesafen in soil，a high efficiency degradation strain BX3 was screened from the activated sludges by enrichment culture． The strain was preliminary identified as Stenotrophomonas acidaminiphila according to its colony morphology，physiological and biochemical characteristics and 16S rDNA gene sequence analysis． Meanwhile， the growth characteristics and degradation characteristics of strain BX3 were preliminary studied． The optimum conditions of fomesafen degradation were as follows: concentration of fomesafen culture solution of 100 mg /L， incubation temperature of 30 ℃ and pH value of 6 - 7． The result shows that strain BX3 had strong fomesafen degradation capacity under the optimum conditions． The removal efficiency of fomesafen could reach more than 80% after incubation of 5 days． The strain BX3 was a suitable candidate in bioremediation of fomesafen-contaminated soil．
Key words:
Fomesafen,
Degradation strain,
Stenotrophomonas acidaminiphila,
Degrading characteristics
中图分类号:
张清明. 氟磺胺草醚降解菌BX3的分离、鉴定与降解特性研究[J]. 华北农学报, 2013, 28(3): 199-203. doi: 10.3969/j.issn.1000-7091.2013.03.036.
Zhang Qingming. Isolation， Identification and Degradation Characteristics of Fomesafen Degradation Bacterium BX3[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2013, 28(3): 199-203. doi: 10.3969/j.issn.1000-7091.2013.03.036.