摘要： 以2个红掌品种为材料, 分别采用愈创木酚法、氮兰四唑(NBT)法、考马斯亮蓝比色法分析研究了红掌叶片愈伤组织诱导和植株再生过程中过氧化物酶(POD)、超氧化物歧化酶(SOD)活性及可溶性蛋白质含量的变化。结果表明: POD和SOD在幼嫩叶片内的活性均较低, 粉冠军的POD活性大于亚历桑娜, SOD活性及可溶性蛋白质含量则低于亚历桑娜; 在愈伤组织诱导阶段, POD和SOD活性及可溶性蛋白质含量都达到最高峰值; 在植株再生过程中, 2种酶及可溶性蛋白质含量都开始下降, 亚历桑娜的POD, SOD活性及可溶性蛋白质含量大于粉冠军; 在小苗移栽后, 酶活性及可溶性蛋白质含量均继续下降至较低水平, 尽管POD和SOD活性仍远高于幼嫩叶片, 但可溶性蛋白质含量却低于幼嫩叶片的水平。
关键词:
红掌,
离体培养,
过氧化物酶,
超氧化物歧化酶,
可溶性蛋白质含量
Abstract: Changes in Peroxidase(POD), Superoxide Dismutase(SOD) activity and soluble protein content during the callus induction and buds differentiation in Anthuraum andraeanum lind were sagtudiedwith Guaiacol process, Nitroblue te-trazolium photoredudion method and Coomassie brilliant blue G-250 respectively. The results showed that POD, SOD and soluble protein content had a very high level in young leaves.However, there had a top level duringcallus induction stage. POD, SOD and soluble protein content start decrease; Activity of POD and SOD of "Arizon" had much higher than that of Pink Champion at the buds differentiation. After the plantlet transplanted, POD and SOD activity and soluble protein content kept low level in the two varieties since POD and SOD activity at this stage had still much higher than that of young leaves, soluble protein content had 1ower than that of young leaves.
Key words:
Anthuraum andraeunum,
In vitro,
Peroxidase(POD),
Superoxide dismutase(SOD) activity,
Soluble protein concern
中图分类号:
夏时云, 麦瑜玲, 林书瀚, 吴国智. 红掌叶片离体培养过程中酶活性及可溶性蛋白质含量的变化[J]. 华北农学报, 2007, 22(6): 195-198. doi: 10.7668/hbnxb.2007.06.040.
XIA Shi-yun, MAI Yu-ling, LIN Shu-han, WU Guo-zhi. Study on Peroxidase, Superoxide Dismutase Activity and Soluble Protein Content Changes During Leaf Culture in vitro of Anthurium andraeanum[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2007, 22(6): 195-198. doi: 10.7668/hbnxb.2007.06.040.