立枯丝核菌不同融合群G蛋白β亚基基因克隆与分子进化分析

doi:10.7668/hbnxb.2017.05.002

华北农学报 ›› 2017, Vol. 32 ›› Issue (5): 7-12. doi: 10.7668/hbnxb.2017.05.002

所属专题： 生物技术；

立枯丝核菌不同融合群G蛋白β亚基基因克隆与分子进化分析

舒灿伟¹, 曹琦琦², 赵美¹, 江绍锋¹, 周而勋¹

1. 华南农业大学农学院, 植物病理学系, 广东省微生物信号与作物病害防控重点实验室, 广东广州 510642;
2. 贵州省出入境检验检疫局, 贵州贵阳 550081

收稿日期:2017-06-21 出版日期:2017-10-28
通讯作者: 周而勋(1963-),男,广东高州人,教授,博士,主要从事植物病原真菌及真菌病害研究。
作者简介:舒灿伟(1985-),男,广东潮安人,讲师,博士,主要从事植物病原真菌及真菌病害研究。
基金资助:
广东省自然科学基金（博士启动）项目（2016A030310454）；国家自然科学基金项目（31470247）；国家公益性行业（农业）科研专项经费项目（No.201403075）

Cloning and Sequence Analysis of G-protein Beta-subunit Genes in Different Anastomosis Groups of Rhizoctonia solani

SHU Canwei¹, CAO Qiqi², ZHAO Mei¹, JIANG Shaofeng¹, ZHOU Erxun¹

1. College of Agriculture, South China Agricultural University, Department of Plant Pathology, Guangdong Province Key Laboratory of Microbial Signals and Disease Control, Guangzhou 510642, China;
2. Guizhou Entry-Exit Inspection and Quarantine Bureau of the People's Republic of China, Guiyang 550081, China

Received:2017-06-21 Published:2017-10-28

摘要/Abstract

摘要： 为了揭示立枯丝核菌不同融合群菌株G蛋白β亚基基因的差异，利用同源克隆的方法，克隆了立枯丝核菌8个融合群共13个菌株的G蛋白β亚基基因，并进行分子进化分析。序列分析结果发现，不同融合群的立枯丝核菌的G蛋白β亚基基因的内含子和开放阅读框数目一致，编码氨基酸均为347个。但氨基酸序列存在14个位点的突变。同源性分析表明，立枯丝核菌各个融合群G蛋白β亚基基因之间的同源性较高，同一亚群的菌株同源性达到100%，不同融合群之间存在差异。分子进化分析表明，不同物种的G蛋白β亚基基因在进化上仍具有一定的保守性，不同融合群菌株的G蛋白β亚基基因序列聚为一个分枝且与同属担子菌门的物种同源性最高。该研究揭示了立枯丝核菌不同融合群G蛋白β亚基基因的特征，为该菌基因功能和分子检测研究奠定了基础。

关键词: 立枯丝核菌, 融合群, G蛋白, 基因克隆, 分子进化

Abstract: To study the genetic difference and molecular evolution of G protein beta-subunit gene of Rhizoctonia solani. Full length of gDNA and cDNA sequences of G protein beta-subunit gene of 13 strains from 8 anastomosis groups (AGs) of Rhizoctonia solani were cloned and analyzed by homology-based cloning method. It showed that the number of intron and open reading frames were consistent in different isolates,which encoded 347 amino acids with 14 point mutations. Homology analysis showed that the homology between AGs of R.solani was high,and the similarity percent of isolates from the same intraspecific group was 100%. But there were genetic diverse among different AGs in homology. The phylogenetic analysis indicated that the G protein beta-subunit gene could effectively distinguish different AGs,and it also could differentiate all the AGs of R.solani from other fungi. It revealed the characteristic of G protein beta-subunit gene in different AGs from R.solani,and laid the foundation for the gene functional analysis and molecular detection of this fungus.

Key words: Rhizoctonia solani, Anastomosis groups, G protein, Gene cloning, Molecular evolution

中图分类号:

舒灿伟, 曹琦琦, 赵美, 江绍锋, 周而勋. 立枯丝核菌不同融合群G蛋白β亚基基因克隆与分子进化分析[J]. 华北农学报, 2017, 32(5): 7-12. doi: 10.7668/hbnxb.2017.05.002.

SHU Canwei, CAO Qiqi, ZHAO Mei, JIANG Shaofeng, ZHOU Erxun. Cloning and Sequence Analysis of G-protein Beta-subunit Genes in Different Anastomosis Groups of Rhizoctonia solani[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2017, 32(5): 7-12. doi: 10.7668/hbnxb.2017.05.002.

http://www.hbnxb.net/CN/Y2017/V32/I5/7

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立枯丝核菌不同融合群G蛋白β亚基基因克隆与分子进化分析

Cloning and Sequence Analysis of G-protein Beta-subunit Genes in Different Anastomosis Groups of Rhizoctonia solani

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立枯丝核菌不同融合群G蛋白β亚基基因克隆与分子进化分析

Cloning and Sequence Analysis of G-protein Beta-subunit Genes in Different Anastomosis Groups of Rhizoctonia solani

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