摘要： 为了研究触角结合蛋白（Antennal binding proteins，ABPs）的生理功能，利用RT-PCR方法克隆了小菜蛾PxylABP基因，并在大肠杆菌中表达，纯化的重组蛋白通过荧光竞争结合试验测定其与29种化合物的结合特性，并运用AutoDock 4.2进行PxylABP蛋白和化合物的分子对接。结果表明，PxylABP与异硫氰酸丙烯酯、己醛、己烷、2-己酮、吲哚和β-紫罗兰酮结合，解离常数分别为0.91，1.81，1.17，2.71，2.74，14.95 μmol/L；分子对接显示，Tyr100与异硫氰酸丙烯酯形成1个氢键。说明PxylABP参与了小菜蛾识别十字花科蔬菜和虫害诱导的植物挥发物的生理过程。

关键词: 小菜蛾, 触角结合蛋白, 荧光竞争结合试验, 分子对接

Abstract: Antennal binding proteins are soluble acidic proteins in insect antenna.It is an important way to clarify ABP physiological function by analysising the binding pattern.The PxylABP gene of Plutella xylostella was cloned using reverse transcription PCR and expressed in Escherichia coli.The specificity of PxylABP binding with 29 compounds was tested through fluorescence competitive binding assay,and the molecular docking of PxylABP with compounds were carried out using AutoDock 4.2.The results showed that PxylABP protein had binding ability to Allylisothiocyanate,Hexane,Hexanal,Indole,2-hexanone and β-Lonone,with the dissociation constants of 0.91,1.81,1.17,2.71,2.74 and 14.95 μmol/L,respcetively;The molecular docking results showed that one hydrogen bond generated between Tyr100 and Allyl-isothiocyanate.These data suggested that PxylABP might be involved in the physiological process that discriminate the cruciferous vegetables and herbivore-induced plant volatiles in Plutella xylostella.

Key words: Plutella xylostella, Antennal binding proteins, Fluorescence competitive binding assay, Molecular docking

中图分类号: 

• Q78