小菜蛾对杀螟丹抗性相关序列的代表性差异分析

doi:10.3321/j.issn:1000-7091.2003.02.004

华北农学报 ›› 2003, Vol. 18 ›› Issue (2): 14-16. doi: 10.3321/j.issn:1000-7091.2003.02.004

小菜蛾对杀螟丹抗性相关序列的代表性差异分析

程罗根¹, 陈之浩², 张晓飞¹, 李忠英²

1. 南京师范大学,生命科学学院,江苏南京 210097;
2. 贵州省农业科学院植保所,贵州贵阳 550006

收稿日期:2002-07-17 出版日期:2003-06-28
作者简介:程罗根(1963-), 男, 安徽安庆人, 博士, 副教授, 硕士生导师, 主要从事遗传学的教学与研究工作.
基金资助:
国家自然科学基金项目(30060051)

Representational Difference Analysis of DNA Sequences Related to Cartap Resistance in Diamondback Moth,Plutella xylostella L.

CHENG Luo gen¹, CHEN Zhi hao², ZHANG Xiao fei¹, LI Zhong ying²

1. College of Life Science, Nanjing Normal University, Nanjing 210097,China;

2. Institute of Plant Protection, Guizhou Academy of Agriculture Science, Guiyang 550006,China

Received:2002-07-17 Published:2003-06-28

摘要/Abstract

摘要： 采用代表性差异分析方法,以小菜蛾的敏感品系作为驱赶扩增子,抗杀螟丹近等基因系作为检测扩增子,通过轮消减杂交得到大小为150～00bp的差异片段,经亚克隆测序并与GenBank等数据库同源比较,发现其中一个亚克隆序列与P450基因有部分同源性,其余亚克隆与已知基因序列的同源性较低,判断这些序列可能是新基因的片段.

关键词: 小菜蛾, 杀螟丹, 代表性差异分析法

Abstract: The genomic difference between Plutella xylostella L.susceptible strain and cartap resistance near isogenic line strain was preliminarily analyzed by representationaLdifference analysis ( RDA)technique.In RDA system, the driver amplicon is susceptible strain while the tester amplicon is cartap resistance near isogenic line strain.After three rounds of subtractive hybridization, the different products were obtained and their sizes were about 150-300 bp.Comparisons between our sub-clone sequencing results and GenBank show that only one sub-clone sequence has P450 gene homology, other sub-clone sequences show no homology to knew gene.Preliminary judgment revealed that these segments might be new gene sequences.

Key words: :Diamondback moth, Plutella xylostella L , Cartap, Representational difference analysis( RDA)

中图分类号:

Q965.9

程罗根, 陈之浩, 张晓飞, 李忠英. 小菜蛾对杀螟丹抗性相关序列的代表性差异分析[J]. 华北农学报, 2003, 18(2): 14-16. doi: 10.3321/j.issn:1000-7091.2003.02.004.

CHENG Luo gen, CHEN Zhi hao, ZHANG Xiao fei, LI Zhong ying. Representational Difference Analysis of DNA Sequences Related to Cartap Resistance in Diamondback Moth,Plutella xylostella L.[J]. ACTA AGRICULTURAE BOREALI-SINICA, 2003, 18(2): 14-16. doi: 10.3321/j.issn:1000-7091.2003.02.004.

http://www.hbnxb.net/CN/Y2003/V18/I2/14

参考文献

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小菜蛾对杀螟丹抗性相关序列的代表性差异分析

Representational Difference Analysis of DNA Sequences Related to Cartap Resistance in Diamondback Moth,Plutella xylostella L.

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小菜蛾对杀螟丹抗性相关序列的代表性差异分析

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