摘要： 为了研究芒果果实中ANS基因的功能。利用同源克隆方法从芒果果实中克隆得到了一个ANS 基因,该基因的cDNA长度为1 252 bp,开放阅读框的长度为1 056 bp,编码351个氨基酸。通过系统发育分析,发现该基因编码的蛋白与荔枝、葡萄、可可豆、桑树等聚在一类。通过序列对比分析表明,已经成功得到芒果ANS基因,并对其生物信息进行了分析,为下一步芒果ANS基因的功能研究打下了基础。

关键词: 芒果, ANS, 基因克隆, 序列分析

Abstract: In order to study the function of ANS gene in mango fruit,a ANS gene was cloned from litchi pericarp by homologous cloning method.The open reading frame was 1 056 bp,and encoding 351 amino acids.The 1 252 bp length fragment was amplified from genome and analysis.It was found that the gene encoding for the protein had close relationship with mountain grape,cocoa,mulberry,and other fruit trees through phylogenetic analysis.

Key words: Mango, ANS, Gene cloning, Sequence analysis

中图分类号: 

• Q78

• S667.7